Synergistic degradation of pyrene by Pseudomonas aeruginosa PA06 and Achromobacter sp. AC15 with sodium citrate as the c
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Synergistic degradation of pyrene by Pseudomonas aeruginosa PA06 and Achromobacter sp. AC15 with sodium citrate as the co-metabolic carbon source Jing Li1,2 Weixin Chen2 Wei Zhou3 Yao Wang2 Maocheng Deng2 Shaoqi Zhou ●
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Accepted: 10 August 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract Two pyrene-degrading strains, Pseudomonas aeruginosa PA06 and Achromobacter sp. AC15 were co-incubated in equal proportions as a microbiological consortium and could enhance the degradation of pyrene. The enzymatic activities of the catechol 1,2-dioxygenase (C12O) and 2,3-dioxygenase activities (C23O) were produced complementary expression by P. aeruginosa PA06 and Achromobacter sp. AC15, respectively. Meanwhile, results showed that pyrene degradation was sufficiently promoted in the presence of sodium citrate as a co-metabolic carbon source, likely a result of enhanced biomass and biosurfactant production. The optimized dosage and ideal initial pHs were 1.4 g L−1 and 5.5, respectively. We also analyzed the rate constant of pyrene degradation, cell growth, and enzyme activity. Results show that P. aeruginosa PA06 had a better effect than Achromobacter sp. AC15 in bacterial growth. However, the C23O or C12O activity produced by Achromobacter sp. AC15 continued at a similar or even faster than that of P. aeruginosa PA06. The mixed bacteria had a better effect than any single bacteria, suggesting the strains worked synergistically to enhance the degradation efficiency. In the co-metabolism system of 600 mg/L pyrene and 1.4 g/L sodium citrate, pyrene degradation reached 74.6%, was 1.57 times, 2.06 times, and 3.89 times that of the mix-culture strains, single PA06 and single AC15 without sodium citrate, respectively. Overall, these findings are valuable as a potential tool for the bioremediation of high-molecular-weight PAHs. Keywords Co-metabolic carbon source Pseudomonas aeruginosa Achromobacter Sodium citrate Pyrene degradation ●
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Introduction
* Maocheng Deng [email protected] * Shaoqi Zhou [email protected] 1
School of Environment and Energy, South China University of Technology, Guangzhou Higher Education Mega Center, Guangzhou 510006, People’s Republic of China
2
School of Food and Biotechnology, Guangdong Industry Polytechnic, Guangzhou 510300, People’s Republic of China
3
Guizhou Academy of Sciences, 1 Shanxi Road, Guiyang 550001, People’s Republic of China
4
State Key Laboratory of Subtropical Building Science, South China University of Technology, Guangzhou 510641, People’s Republic of China
Polycyclic aromatic hydrocarbons (PAHs) are common contaminants associated with the petrochemical industry and can be found in other sources such as tobacco smoke (Nzila 2018a). These compounds are ubiquitous in the environment and consist of two or more fused benzene rings. PAHs represent a class of substances listed as human health hazards with toxic, carcinogenic, and mutagenic properties by the International Agency for Researc
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