Synthetic Metabolic Pathways Methods and Protocols
This volume outlines key steps associated with the design, building, and testing of synthetic metabolic pathways for optimal cell factory performance and robustness, and illustrates how data-driven learning from these steps can be used for rational c
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Michael Krogh Jensen Jay D. Keasling Editors
Synthetic Metabolic Pathways Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Synthetic Metabolic Pathways Methods and Protocols
Edited by
Michael Krogh Jensen The Novo Nordisk Foundation Center for Biosustainability, Technical University of Denmark, Kongens Lyngby, Denmark
Jay D. Keasling Joint BioEnergy Institute, Emeryville, CA, USA
Editors Michael Krogh Jensen The Novo Nordisk Foundation Center for Biosustainability Technical University of Denmark Kongens Lyngby, Denmark
Jay D. Keasling Joint BioEnergy Institute Emeryville, CA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7294-4 ISBN 978-1-4939-7295-1 (eBook) https://doi.org/10.1007/978-1-4939-7295-1 Library of Congress Control Number: 2017957981 © Springer Science+Business Media, LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Biobased conversion of renewable carbon sources into products related to health, food, and transportation is growing, and so is the industry evolved around it. The enormous interest in this organism industry relates to the accomplishments within metabolic engineering during the last 25 years, and the more recent adoption of biological research as an engineering discipline collectively referred to as synthetic biology. Both metabolic engineering and synthetic biology scale with reduced costs of DNA synthesis and sequencing, and by way of this the t
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