Tertiary Lymphoid Structures Methods and Protocols
This volume explores the various methods used to study tertiary lymphoid structures (TLS) in pathological situations. Pre-clinical models are also discussed in detail to show how TLS structure, development, and maintenance can be targeted and studied in v
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Marie-Caroline Dieu-Nosjean Editor
Tertiary Lymphoid Structures Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Tertiary Lymphoid Structures Methods and Protocols
Edited by
Marie-Caroline Dieu-Nosjean Laboratory “Cancer, Immune Control and Escape”, UMRS 1138 INSERM, Cordeliers Research Center, Sorbonne University, UMR 1138, Paris Descartes University, Sorbonne Paris City, UMR 1138, Paris, France
Editor Marie-Caroline Dieu-Nosjean Laboratory “Cancer, Immune Control and Escape” UMRS 1138 INSERM Cordeliers Research Center Sorbonne University, UMR 1138 Paris Descartes University Sorbonne Paris City, UMR 1138 Paris, France
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8708-5 ISBN 978-1-4939-8709-2 (eBook) https://doi.org/10.1007/978-1-4939-8709-2 Library of Congress Control Number: 2018950391 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The immune system has developed many mechanisms to build up a protective response against pathogens or abnormal cells. Some of them take place in organs (i.e. secondary lymphoid organs, SLO) or structures (such as gut-associated lymphoid tissue (GALT) and mucosa-associated lymphoid tissue (MALT)) that are lifelong and fully dedicated to the elaboration of adaptive immune responses. However, there are also inducible lymphoid structures that develop transiently in response to chronic infl
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