The mitochondrial genome of the Microhyla taraiensis (Anura: Microhylidae) and related phylogenetic analyses
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TECHNICAL NOTE
The mitochondrial genome of the Microhyla taraiensis (Anura: Microhylidae) and related phylogenetic analyses Janak Raj Khatiwada1,2 · Shouhong Wang1,2 · Guocheng Shu1,2 · Feng Xie1 · Jianping Jiang1
Received: 30 July 2017 / Accepted: 11 August 2017 © Springer Science+Business Media B.V. 2017
Abstract The complete mitochondrial genome of the Microhyla taraiensis from Eastern Nepal was sequenced. The genome has a typical gene order identical to most of other anuran species. The entire mtDNA of this species, 16,764 bp in length, containing 13 protein-coding genes, 2 rRNA genes, 22 tRNA genes, and a control region. Phylogenetic relationships was constructed based on the complete mitogenome of M. taraiensis and other species of Microhylidae, using Bayesian inferences and Maximum likelihood approaches, and supports the monophly for this family. This work contributes to basic molecular data which is critical for further genetic research and conservation of this newly described species. Keywords Microhylidae · Microhyla taraiensis · Mitogenome · Nepal The microhylid frogs genus Microhyla Tschudi, 1838, show a broad distributional range in Asia, from Japan, China, South Asia and throughout Southeast Asia to Sumatra, Borneo, Java, and Bali (Frost 2017). Recently described Microhyla taraiensis is only known from Jamun Khadi, Jhapa district, Eastern Nepal and listed as Data Deficient (DD) species (Khatiwada et al. 2017). Therefore, complete Janak Raj Khatiwada and Shouhong Wang have contributed equally to this work. * Jianping Jiang [email protected] 1
Chengdu Institute of Biology, Chinese Academy of Sciences, Chengdu 610041, China
University of Chinese Academy of Sciences, Beijing 100049, China
2
mitochondrial genome would improve our understanding for further genetic research and conservation of this newly described species. In this study, DNA fragments of M. taraiensis was extracted from clipped toes preserved in 95% ethanol using the DNeasy Tissue Kit (QIAGEN) from a single specimen of M. taraiensis collected from Jamun Khadi, (26.65358°N and 87.91161°E; elevation 119 m asl) in May 2015. The DNA fragments were amplified and subjected to polymerase chain reaction (PCR) with primers as described by Zhang et al. (2013). The amplified PCR products were purified using a Qiagen PCR purification kit, and sequences were obtained from an ABI 3100 automated sequencer. Newly determined sequence was deposited in GenBank Accession No. MF496241. The complete mitogenome of M. taraiensis (Fig. 1) has a total length of 16,764 bp and contains 37 genes, including 13 protein-coding genes, 2 ribosomal RNA (12S rRNA and 16S rRNA) genes, and 22 transfer RNA (tRNA) genes and a control region (D-loop) (Table 1). The mtDNA gene organization of M. taraiensis was roughly similar with other Microhylidae (Deng et al. 2016; Wang et al. 2016; Wu et al. 2016). The overall base composition of the complete mitochondrial genome was A: 29.7%, C: 24.6%, G: 14.0% and T: 31.7%. The base composition of A+T (61.38%) was higher than
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