The Nuclear Envelope Methods and Protocols
This volume provides a wide range of protocols used in studying the nuclear envelope, with special attention to the experimental adjustments that may be required to successfully investigate this complex organelle in cells from various organisms. The Nucle
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Sue Shackleton Philippe Collas Eric C. Schirmer Editors
The Nuclear Envelope Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
The Nuclear Envelope Methods and Protocols
Edited by
Sue Shackleton Department of Biochemistry, University of Leicester, Leicester, UK
Philippe Collas Institute of Basic Medical Sciences, University of Oslo, Oslo, Norway
Eric C. Schirmer Wellcome Trust Centre for Cell Biology, University of Edinburgh, Edinburgh, UK
Editors Sue Shackleton Department of Biochemistry University of Leicester Leicester, UK
Philippe Collas Institute of Basic Medical Sciences University of Oslo Oslo, Norway
Eric C. Schirmer Wellcome Trust Centre for Cell Biology University of Edinburgh Edinburgh, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3528-4 ISBN 978-1-4939-3530-7 (eBook) DOI 10.1007/978-1-4939-3530-7 Library of Congress Control Number: 2016937417 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: Super-resolution light microscopy reconstruction of a nucleus from a cultured human dermal fibroblast accumulating immature lamin A, labelled to show prelamin A (green), mature lamin A/C (orange) and DNA (blue). For further information, see Chapter 10. Image credit of Marek Drozdz and David Vaux. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface The nuclear envelope (NE) is a double membrane system enclosing the nucleus and is the central distinguishing feature of all eukaryotes. In addition to protecting the genetic material, the NE regulates the trafficking of proteins, RNAs, and ribosomes between the nucleus and cytoplasm. More recently, the iden
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