Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids
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ARTICLE
POLYMER SCIENCE
https://doi.org/10.1007/s10118-020-2452-4 Chinese J. Polym. Sci.
Thermoresponsive Nanogels from Dendronized Copolymers for Complexation, Protection and Release of Nucleic Acids Yi Yao, Jian-Hui Wu, Shi-Jie Cao, Bi-Yi Xu, Jia-Tao Yan, Di Wu, Wen Li*, and Afang Zhang* Department of Polymer Materials, School of Materials Science & Engineering, Shanghai University, Shanghai 200444, China
Electronic Supplementary Information Abstract A series of thermoresponsive cationic dendronized copolymers and their corresponding nanogels containing dendritic oligoethylene glycol (OEG) units and guanidine groups were prepared, and their complexation, protection, and release of nucleic acids were investigated. The dendritic OEGs endow these copolymer materials with good biocompatibility and characteristic thermoresponsiveness, while cationic guanidine groups can efficiently bind with the nucleic acids. The dendritic topology also affords the copolymers specific shielding effect which plays an essential role in protecting the activity of nucleic acids. At room temperature, dendronized copolymers and the corresponding nanogels could efficiently capture and condense the nucleic acids, while above their cloud points (Tcps), more than 75% of siRNA could be released in 1 h triggered by ATP. More importantly, the copolymer showed protective capability to siRNA, while nanogels exhibit even better protection when compared to the copolymers due to the synergetic effect from the three-dimensional cross-linked network and high density of dendritic units in vicinity. This kind of smart dendronized copolymer nanogels form a novel class of scaffolds as promising materials for biomedical applications. Keywords Dendronized polymer; Guanidine; Nanogels; Thermoresponsive; Bioactive protection Citation: Yao, Y.; Wu, J. H.; Cao, S. J.; Xu, B. Y.; Yan, J. T.; Wu, D.; Li, W.; Zhang, A. Thermoresponsive nanogels from dendronized copolymers for complexation, protection and release of nucleic acids. Chinese J. Polym. Sci. https://doi.org/10.1007/s10118-020-2452-4
INTRODUCTION Biomacromolecules, including proteins, peptides, and nucleic acids, play pivotal roles in a range of biological processes, and can be effective components of drugs for a variety of diseases.[1] However, most of them can lose their biological activity easily in extracellular environments.[2] Recent researches suggest that formation of hybrid conjugates with synthetic polymer materials via covalent and/or non-covalent methods is an efficient way to improve the stability of these bioactives.[3] In particular, modification via supramolecular interactions such as electrostatic interactions can not only enhance the stability of proteins but also provide possibility of releasing biomacromolecules from the polymers under the designated conditions, for instance, with temperature,[4] pH,[5] redox,[6] or ATP[7] as stimulus. A variety of polymer materials have been developed as conjugated matrix, such as hyperbranched polymers,[8] dendrimers,[9] polymer brus
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