Coralloluteibacterium stylophorae gen. nov., sp. nov., a new member of the family Lysobacteraceae isolated from the reef
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ORIGINAL PAPER
Coralloluteibacterium stylophorae gen. nov., sp. nov., a new member of the family Lysobacteraceae isolated from the reef-building coral Stylophora sp. Wen‑Ming Chen1 · Pei‑Bei Xie2 · Sen‑Lin Tang3 · Shih‑Yi Sheu2 Received: 21 March 2017 / Revised: 14 November 2017 / Accepted: 16 November 2017 / Published online: 30 November 2017 © Springer-Verlag GmbH Germany, part of Springer Nature 2017
Abstract A bacterial strain, designated Sty a-1T, was isolated from a reef-building coral Stylophora sp., collected off coast of Southern Taiwan and characterized using the polyphasic taxonomy approach. Cells of strain Sty a-1T were Gram-staining-negative, aerobic, poly-β-hydroxybutyrate accumulating, motile by means of flagella, non-spore forming, straight rod-shaped and colonies were yellow and circular. Growth occurred at 15–40 °C (optimum, 30–35 °C), at pH 6–10 (optimum, pH 6.5–8) and with 0–7% NaCl (optimum, 2–3%). The predominant fatty acids were iso-C15:0, iso-C17:1ω9c, summed feature 3 (comprising C16:1ω7c and/or C16:1ω6c) and iso-C17:0. The major isoprenoid quinone was Q-8 and the DNA G+C content was 68.5 mol%. The polar lipid profile consisted of a mixture of phosphatidylethanolamine, phosphatidylglycerol, phosphatidylcholine, diphosphatidylglycerol, an uncharacterized aminophospholipid and three uncharacterized lipids. The major polyamines were spermidine, putrescine and homospermidine. Phylogenetic analyses based on 16S rRNA and four housekeeping gene sequences (recA, atpD, rpoA and rpoB) showed that strain Sty a-1T forms a distinct lineage with respect to closely related genera in the family Lysobacteraceae, most closely related to Lysobacter, Silanimonas, Arenimonas and Luteimonas and the levels of 16S rRNA gene sequence similarity with respect to the type species of related genera are less than 95%. On the basis of the genotypic and phenotypic data, strain Sty a-1T represents a novel genus and species of the family Lysobacteraceae, for which the name Coralloluteibacterium stylophorae gen. nov., sp. nov. is proposed. The type strain is Sty a-1T (= BCRC 80968T = LMG 29479T = KCTC 52167T). Keywords Coralloluteibacterium stylophorae gen. nov., Sp. nov. · Reef-building coral · Stylophora sp. · Polyphasic taxonomy
Introduction Communicated by Erko Stackebrandt. The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA, recA, atpD, rpoA and rpoB gene sequences of Coralloluteibacterium stylophorae strain Sty a-1T are LN997852, MG197787, MG197788, MG197789 and MG197790, respectively. The Digital Protologue database Taxon Numbers for genus Coralloluteibacterium and strain Sty a-1T is GA00050 and TA00331, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-017-1458-y) contains supplementary material, which is available to authorized users. * Shih‑Yi Sheu [email protected] Extended author information available on the last page of the article
The family Lysobacteraceae (type genus, Lysobacter), originally proposed by Christensen and Co
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