Vallicoccus soli gen. nov., sp. nov., a novel actinobacterium isolated from soil, and description of Vallicoccaceae fam.
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ORIGINAL PAPER
Vallicoccus soli gen. nov., sp. nov., a novel actinobacterium isolated from soil, and description of Vallicoccaceae fam. nov., Motilibacterales ord. nov. Yu-Zhou Feng . Wei-Xun Chunyu . Rui Liang . Richard L. Hahnke . Peter Schumann . Yu-Rong Zhao . Sheng Gao . Min Yin . Yang Ji . Le Sun . Xiao-Yang Zhi . Shu-Kun Tang
Received: 12 August 2020 / Accepted: 13 October 2020 Ó Springer Nature Switzerland AG 2020
Abstract A novel Gram-stain-positive, aerobic, cocci-shaped actinobacterium, designated YIM 75000T, was isolated from a soil sample collected from a dry-hot river valley in Yunnan Province, P.R. China. Growth was observed at 10–45 °C (optimal 37 °C), 0–8% (w/v) NaCl (optimal at 0–3% NaCl) and pH 6.0–8.0 (optimal at pH 7.3). The peptidoglycan contained LL-diaminopimelic acid, glycine, glutamic acid as well as alanine and its type was A3c with an LL-Dpm-Gly interpeptide bridge. The major cellular Yu-Zhou Feng, Wei-Xun Chunyu and Rui Liang contributed equally to this work. The GenBank/EMBL/DDBJ accession number for the 16S rRNA gene sequence of strain YIM 75000T is JN188954. The Whole genome of strain YIM 75000T has been deposited at DDBJ/ENA/GenBank under the accession number QZEZ00000000.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10482-020-01484-5) contains supplementary material, which is available to authorized users. Y.-Z. Feng W.-X. Chunyu Y.-R. Zhao S. Gao Y. Ji X.-Y. Zhi (&) S.-K. Tang (&) Yunnan Institute of Microbiology, Key Laboratory for Conservation and Utilization of Bio-resource, and Key Laboratory for Microbial Resources of the Ministry of Education, School of Life Sciences, Yunnan University, Kunming 650091, People’s Republic of China e-mail: [email protected]
fatty acids ([ 10%) were C16:0, Summed In Feature 3 (C16:1 x6c/C16:1 x7c) and C17:1 x8c. The predominant menaquinone was MK-9(H4). The major whole-cell sugars contained rhamnose, ribose, arabinose and mannose. The DNA G?C content was 77.0 mol%. The 16S rRNA gene sequence similarities of strain YIM 75000T with other species were less than 94%. Phylogenetic analyses based on 16S rRNA gene sequences and genome data, revealed that strain YIM 75000T together with the genus Motilibacter formed a distinct phylogenetic lineage within the phylum Actinobacteria, separating them from members of all orders. Strain YIM 75000T showed 73.4–73.7% average nucleotide identity and 19.5–19.7% digital DNA–DNA hybridization identity with the closely related genus Motilibacter. Based on the phenotypic, phylogenetic and chemotaxonomic data, it is proposed that the new isolate represents the nomenclature type of the novel species Vallicoccus soli gen. nov., sp. nov. (YIM 75000T = DSM 45377T= KCTC 49228T = CGMCC 1.13844T) which is the nomenclature type of the novel genus Vallicoccus gen. S.-K. Tang e-mail: [email protected] Y.-Z. Feng West China Biomedical Big Data Center, West China Hospital, Sichuan University, Chengdu 610065, People
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