Utilization of mixed fruit waste for exopolysaccharide production by Bacillus species SRA4: medium formulation and its o
- PDF / 1,104,228 Bytes
- 9 Pages / 595.276 x 790.866 pts Page_size
- 39 Downloads / 151 Views
ORIGINAL ARTICLE
Utilization of mixed fruit waste for exopolysaccharide production by Bacillus species SRA4: medium formulation and its optimization Avni Vaishnav1 · Kinjal Upadhayay1 · Devayani Tipre2 · Shailesh Dave3 Received: 10 April 2020 / Accepted: 6 November 2020 / Published online: 24 November 2020 © King Abdulaziz City for Science and Technology 2020
Abstract The main focus of this research work was to carry out the fermentative production of EPS with mixed fruit waste as substrate. The medium formulation studied by sequential addition of medium components and replacement of sugar with mixed fruit waste. Amongst the six species of Bacillus studied, Bacillus species SRA4 produced EPS in range of 3.0–17.9 g/L, which was highest amongst all the species selected for the study. Thus for further optimization, the response surface methodology was used making use of Bacillus species SRA4. EPS production enhanced to 23.75 g/L. In 10 L shake flask and fermenter scale-up study was carried out. In the study, 10 L flask showed the highest EPS production of 17.95 g/L in 120 h, whereas in 10 L fermenter, it was as high as 25.1 g/L that too in 72 h only. The optimization study resulted in 1.76-fold increase in EPS production with nearly 48 h reduction in EPS production time as compared to initial production procedure. Replacement of sucrose with fruit waste extract made the process environmentally friendly; omission of l-cystine and use of 50% reduced amount of sodium acetate in the medium lowered the production cost of EPS. This method also solved the fruit waste disposal problem. Keywords Bacillus · Exopolysaccharide (EPS) · Mixed fruit waste extract (MFWE) · Scale-up · Ooptimization · Response surface methodology (RSM)
Introduction Increased demand for natural polymers for biotechnological, medicinal, cosmetics, food, pharmaceuticals and other industrial applications has led to a remarkable interest in polysaccharides produced by microorganisms (Dave et al. 2016). Thus, substantial interest has aroused with regard to the isolation and identification of new microbial polysaccharides that might have innovative applications as emulsifier, gelling and stabilizing agent (Sutherland 2001). A vast number of microorganisms produce exopolysaccharide (EPS) as a strategy of growth, surviving under adverse conditions * Shailesh Dave [email protected] 1
Department of Biochemistry and Biotechnology, Saint Xavier’s Collage, Ahmedabad, India
2
Department of Microbiology, School of Science, Gujarat University, Ahmedabad, India
3
Loyola Centre for Research and Development, Xavier’s Research Foundation, Saint Xavier’s Collage Campus, Ahmedabad, India
and adhering to solid surfaces. The functional role of EPS depends on the ecological niches and the natural environment from which microorganisms have been isolated. EPS production is an energy cost-intensive process, 70% of the cost is for the carbon source required for the production (Poli et al. 2011). To cut down the cost of production, several researchers hav
Data Loading...
