Vaccine Design Methods and Protocols: Volume 1: Vaccines for Human D
This text provides a practical guide providing step-by-step protocol to design and develop vaccines. Chapters detail protocols for developing novel vaccines against infectious bacteria, viruses, fungi, and parasites for humans and animals. Volume 1: Vacci
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Sunil Thomas Editor
Vaccine Design Methods and Protocols Volume 1: Vaccines for Human Diseases
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Vaccine Design Methods and Protocols: Volume 1: Vaccines for Human Diseases
Edited by
Sunil Thomas Lankenau Institute for Medical Research, Philadelphia, PA, USA
Editor Sunil Thomas Lankenau Institute for Medical Research Philadelphia, PA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3385-3 ISBN 978-1-4939-3387-7 (eBook) DOI 10.1007/978-1-4939-3387-7 Library of Congress Control Number: 2016934046 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Dedication Dedicated to Vaccinologists (who work hard developing vaccines) Healthcare workers in developing countries (who risk their lives vaccinating people)
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Preface “We are protecting children from polio at the cost of our lives.” Sabeeha Begum (a lady healthcare worker providing polio vaccines in Quetta, Pakistan)
Vaccinations have helped in preventing several diseases; however, as yet, there are only two diseases that have been eradicated globally. Mass awareness programs and aggressive vaccination strategies in the twentieth century were able to control smallpox, and the disease was officially declared eradicated in 1980. Rinderpest, a serious disease of cattle, was officially eradicated in 2011, thereby becoming only the second disease to be completely eradicated. Recently, the Americas (North and South America) were declared free of endemic transmission of rubella, a contagious viral disease tha
