Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived i
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(2020) 14:23
RESEARCH
Open Access
Alginate/Pluronic F127-based encapsulation supports viability and functionality of human dental pulp stem cell-derived insulin-producing cells Suryo Kuncorojakti1,2, Watchareewan Rodprasert2, Supansa Yodmuang3,4, Thanaphum Osathanon5,6, Prasit Pavasant5,6, Sayamon Srisuwatanasagul7* and Chenphop Sawangmake2,8,9*
Abstract Background: Current approach for diabetes treatment remained several adverse events varied from gastrointestinal to life-threatening symptoms. Regenerative therapy regarding Edmonton protocol has been facing serious limitations involving protocol efficiency and safety. This led to the study for alternative insulin-producing cell (IPC) resource and transplantation platform. In this study, evaluation of encapsulated human dental pulp-derived stem cell (hDPSC)-derived IPCs by alginate (ALG) and pluronic F127-coated alginate (ALGPA) was performed. Results: The results showed that ALG and ALGPA preserved hDPSC viability and allowed glucose and insulin diffusion in and out. ALG and ALGPA-encapsulated hDPSC-derived IPCs maintained viability for at least 336 h and sustained pancreatic endoderm marker (NGN3), pancreatic islet markers (NKX6.1, MAF-A, ISL-1, GLUT-2 and INSULIN), and intracellular pro-insulin and insulin expressions for at least 14 days. Functional analysis revealed a glucoseresponsive C-peptide secretion of ALG- and ALGPA-encapsulated hDPSC-derived IPCs at 14 days post-encapsulation. Conclusion: ALG and ALGPA encapsulations efficiently preserved the viability and functionality of hDPSC-derived IPCs in vitro and could be the potential transplantation platform for further clinical application. Keywords: Insulin-producing cells (IPCs), Dental pulp stem cells (DPSCs), Diabetes mellitus, Encapsulation, Alginate, Pluronic F127
Background Diabetes mellitus is an intractable metabolic disease. Epidemiological studies covering 2.7 million of participants during 1980–2008 reported the global increases of glycemia and diabetes prevalence which were correlated * Correspondence: [email protected]; [email protected]; [email protected]; [email protected] 7 Department of Veterinary Anatomy, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand 2 Veterinary Stem Cell and Bioengineering Innovation Center (VSCBIC), Veterinary Pharmacology and Stem Cell Research Laboratory, Faculty of Veterinary Science, Chulalongkorn University, Bangkok 10330, Thailand Full list of author information is available at the end of the article
with population growth and ageing [1]. Moreover, the global prevalence of diabetes is expected to be 693 million in all age-group worldwide by 2045 [2]. Several approaches have been clinically introduced to manage hyperglycemic conditions and consequence complications i.e. exogenous insulin and pharmacotherapeutic preparations [3]. However, there were some reports suggested an evidence of hypoglycemia and adverse events [4]. From these reasons, many of researchers have been trying in finding novel approach to cope dia
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