Antibiotic Resistance Protocols
This third edition provides a wide range of different technologies, ranging from conventional growth basic techniques, application of molecular biology, development of resistance mutations, and diagnosis and monitoring treatment response. New and up
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Stephen H. Gillespie Editor
Antibiotic Resistance Protocols Third Edition
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Antibiotic Resistance Protocols Third Edition
Edited by
Stephen H. Gillespie School of Medicine, University of St. Andrews, North Haugh, UK
Editor Stephen H. Gillespie School of Medicine University of St. Andrews North Haugh, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7636-2 ISBN 978-1-4939-7638-6 (eBook) https://doi.org/10.1007/978-1-4939-7638-6 Library of Congress Control Number: 2017963343 © Springer Science+Business Media, LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Since the first two editions in this series were published, much has changed in the world of antibiotic resistance research. Importantly, national and international reports have highlighted the cost of antibiotics resistance to world health. There is a recognition of the need to understand the processes that drive resistance and to identify ways to ameliorate the apparently inexorable progress to a post-antibiotic age. At the same time, our science has not stood still and there are new tools to address the old questions. This new edition of Antibiotic Resistance Protocols draws on a wide range of different technologies. These range from conventional growth-based techniques to the application of molecular biology to understand the development of resistance mutations, diagnosis, and monitoring tr
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