Antibiotics Methods and Protocols
This volume provides state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Antibiotics: Methods
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Peter Sass Editor
Antibiotics Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Antibiotics Methods and Protocols
Edited by
Peter Sass Interfaculty Institute for Microbiology and Infection Medicine, Microbial Bioactive Compounds, University of Tübingen, Tübingen, Germany
Editor Peter Sass Interfaculty Institute for Microbiology and Infection Medicine Microbial Bioactive Compounds University of Tübingen Tübingen, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6632-5 ISBN 978-1-4939-6634-9 (eBook) DOI 10.1007/978-1-4939-6634-9 Library of Congress Control Number: 2016956545 © Springer Science+Business Media New York 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A
Preface The increasing prevalence of antibiotic-resistant microbes challenges modern medicine, posing serious threats to human and animal health. Therefore, we desperately need new antibiotics with novel mechanisms of action and resistance-breaking properties. This edition of Antibiotic Research Protocols in the Springer series Methods in Molecular Biology aims to provide state-of-the-art and novel methods on antibiotic isolation and purification, identification of antimicrobial killing mechanisms, and methods for the analysis and detection of microbial adaptation strategies. Accordingly, the chapters are organized under three major themes: Production and Design, Mode of Action, and Response and Susceptibility. In the first part on antibiotic production and design, contributions report on strategies to find new antibacterial compounds by mini
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