Assessment of genetic diversity and population structure among a collection of Korean Perilla germplasms based on SSR ma
- PDF / 1,177,997 Bytes
- 12 Pages / 595.276 x 790.866 pts Page_size
- 96 Downloads / 193 Views
Genes & Genomics https://doi.org/10.1007/s13258-020-01013-8
RESEARCH ARTICLE
Assessment of genetic diversity and population structure among a collection of Korean Perilla germplasms based on SSR markers Jun Seok Oh1 · Kyu Jin Sa1 · Do Yoon Hyun2 · Gyu‑Taek Cho2 · Ju Kyong Lee1 Received: 4 June 2020 / Accepted: 16 October 2020 © The Genetics Society of Korea 2020
Abstract Background Information on the genetic variation of genetic resource collections is very important for both the conservation and utilization of crop germplasms in genebanks. Var. frutescens of Perilla crop is extensively cultivated in South Korea as both an oil crop and a vegetable crop. Objectives We used SSR markers to evaluate the genetic diversity, genetic relationships, and population structure of 155 accessions of var. frutescens that have been selected as genetic resources for the development of leaf vegetable cultivars and preserved in the RDA-Genebank collection from South Korea. Methods A total of 155 accessions of var. frutescens of Perilla crop collected in South Korea were obtained from the RDA-Genebank of the Republic of Korea. We selected 20 SSR markers representing the polymorphism of and adequately amplifying all the Perilla accessions. Results The average GD and PIC values were 0.642 and 0.592, respectively, with ranges of 0.244–0.935 and 0.232– 0.931. The genetic variability in the southern region of South Korea was higher than that in the central region. The clustering patterns were not clearly distinguished between the accessions of var. frutescens from the central and southern regions of South Korea. Conclusion These results regarding the genetic diversity and population structure of the 155 accessions of var. frutescens of South Korea provide useful information for understanding the genetic variability of this crop and selecting and managing core germplasm sets in the RDA-Genebank of the Republic of Korea. Keywords Perilla crop · Geographical variation · Gene diversity · SSR marker · Population structure
Introduction Perilla frutescens (L.) Britt. (Lamiaceae) is a self-fertilizing crop that is divided into two varieties (or cultivated types) based on morphology and use in East Asia. P. frutescens var. frutescens is used as an oil crop or vegetable crop (Dlggae in Korean, Egoma in Japanese, Ren in Chinese), while P. Jun Seok Oh, Kyu Jin Sa have contributed equally to this work. * Ju Kyong Lee [email protected] 1
Department of Applied Plant Sciences, College of Agriculture and Life Sciences, Kangwon National University, Chuncheon 24341, South Korea
National Agrobiodiversity Center, National Institute of Agricultural Sciences, RDA, Jeonju 54874, South Korea
2
frutescens var. crispa is used a Chinese medicine or vegetable crop (Cha-jo-ki in Korean, Shiso in Japanese, Zisu in Chinese). In East Asia, these two varieties of Perilla crop are cultivated as separate crops in each country. The two cultivated types of Perilla crop are classified according to their morphological characteristics as follows: P. fr
Data Loading...
