Bacillus alkalicellulosilyticus sp. nov., isolated from extremely alkaline bauxite residue (red mud) site
- PDF / 736,249 Bytes
- 5 Pages / 595.276 x 790.866 pts Page_size
- 99 Downloads / 212 Views
ORIGINAL PAPER
Bacillus alkalicellulosilyticus sp. nov., isolated from extremely alkaline bauxite residue (red mud) site Guo‑Hong Liu1 · Manik Prabhu Narsing Rao2 · Xiao‑Ying Wang1 · Tong‑Wei Chu3 · Bo Liu1 · Wen‑Jun Li2,4 Received: 9 June 2020 / Revised: 28 August 2020 / Accepted: 30 September 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A Gram-stain-negative, rod-shaped, facultatively anaerobic, motile and spore-forming strain designated FJAT-44921T was isolated from red mud collected from Chiping County, Shandong Province, China. The 16S rRNA gene sequence result showed that strain FJAT-44921T shared a low sequence identity (96.6%) with the members of the genus Bacillus. Growth was observed at pH 8.0–10.0 (optimum pH 9.0), 10–40 °C (optimum 20–25 °C) with 0–8% (v/w %) NaCl (optimum 4–6 v/w %). FJAT-44921T consists of MK-7 as the isoprenoid quinone and meso-2,6-diaminopimelic acid as the cell-wall diamino acid. The predominant fatty acids were anteiso-C15:0, iso-C15:0, C16:0, and anteiso-C17:0. The polar lipids were diphosphatidylglycerol, phosphatidyl glycerol, phosphatidylmethylethanolamine, unidentified phospholipid, and unidentified aminophospholipid. The genomic DNA G + C content was 37.3 mol%. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between FJAT-44921T and other closely related Bacillus members were lower than the recognized threshold values of ANI (95–96%) and dDDH (70%) recommended as the criterion for interspecies identity. The type strain is FJAT-44921T (=CCTCC AB 2016196T =DSM 104630T). Keywords Bacillus alkalicellulosilyticus sp. nov. · Alkaliphilic · Red mud · Cellulolytic
Introduction Communicated by Erko stackebrandt. Guo-Hong Liu and Manik Prabhu Narsing Rao Contributed equally to the work. The GenBank/ENA/DDBJ accession numbers for 16S rRNA gene and genome sequence of strain FJAT-44921T were KY640613 and MTIY00000000, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02063-y) contains supplementary material, which is available to authorized users. * Bo Liu [email protected] * Wen‑Jun Li [email protected] 1
Agricultural Bio‑Resources Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350003, PR China
State Key Laboratory of Biocontrol and Guangdong Provincial Key Laboratory of Plant Resources, School of Life Sciences, Sun Yat-Sen University, Guangzhou 510275, PR China
2
Bauxite residue (red mud) is a worldwide problem (Graefe and Klauber 2011) which is generated as a by-product during the extraction of alumina from bauxite ore (Krishna et al. 2014). It is often disposed of in nearby engineered impoundments and conditions in these regions are extreme, characterized by high pH, high concentrations of soluble ions with low or virtually no organic matter (Krishna et al. 2014; Gadd and Griffiths 1977). During evaluating the bacterial diversity of such environment, strain designated FJAT44921T was isolated. The 16S rRNA gen
Data Loading...
