Aequoribacter fuscus gen. nov., sp. nov., a new member of the family Halieaceae , isolated from coastal seawater
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Aequoribacter fuscus gen. nov., sp. nov., a new member of the family Halieaceae, isolated from coastal seawater§ Shan-Hui Li, Jaeho Song, Ilnam Kang, Juchan Hwang, and Jang-Cheon Cho* Department of Biological Sciences, Inha University, Incheon 22212, Republic of Korea (Received Apr 17, 2020 / Revised May 4, 2020 / Accepted May 4, 2020)
A Gram-stain-negative, rod-shaped, obligately aerobic, nonflagellated, and chemoheterotrophic bacterium, designated IMCC3088T, was isolated from coastal seawater of the Yellow Sea. The 16S rRNA gene sequence analysis indicated that this strain belonged to the family Halieaceae which shared the highest sequence similarities with Luminiphilus syltensis NOR5-1BT (94.5%) and Halioglobus pacificus S1-72T (94.5%), followed by 92.3–94.3% sequence similarities with other species within the aforementioned family. Phylogenetic analyses demonstrated that strain IMCC3088T was robustly clustered with Luminiphilus syltensis NOR5-1BT within the family Halieaceae. However, average amino acid identity (AAI), percentages of conserved proteins (POCP), average nucleotide identity (ANI), and alignment fraction (AF) between strain IMCC3088T and Luminiphilus syltensis NOR5-1BT were 54.5%, 47.7%, 68.0%, and 16.5%, respectively, suggesting that they belonged to different genera. Whole-genome sequencing of strain IMCC3088T revealed a 3.1 Mbp genome size with a DNA G + C content of 51.7 mol%. The genome encoded diverse metabolic pathways including sulfur oxidation, phenol degradation, and proteorhodopsin phototrophy. Mono-unsaturated fatty acids were found to be the predominant cellular fatty acid components in the strain. Phosphatidylethanolamine, phosphatidylglycerol, and diphosphatidylglycerol were the primarily identified polar lipids, and ubiquinone-8 was identified as a major respiratory quinone. The taxonomic data collected herein suggested that strain IMCC3088T represented a novel genus and species of the family Halieaceae, for which the name Aequoribacter fuscus gen. nov., sp. nov. is proposed with the type strain (= KACC 15529T = NBRC 108213T). Keywords: Aequoribacter fuscus, taxonomy, complete genome, Halieaceae, coastal sea, marine surface
*For correspondence. E-mail: [email protected]; Tel.: +82-32-860-7711; Fax: +82-32-232-0541 § Supplemental material for this article may be found at http://www.springerlink.com/content/120956. Copyright G2020, The Microbiological Society of Korea
Introduction The family Halieaceae (Spring et al., 2015), previously known as the OM60/NOR5 clade of the oligotrophic marine Gammaproteobacteria (OMG) group (Cho and Giovannoni, 2004), belongs to the order Cellvibrionales in the class Gammaproteobacteria and was established through a genome-scale study on the OMG group (Spring et al., 2015). As of April 2020, the family comprises at least 11 genera exclusively isolated from marine environments, including Haliea (Urios et al., 2008; Lin et al., 2015; Yang et al., 2020), Congregibacter (Spring et al., 2009), Chromatocurvus (Csotonyi et al.
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