Bioluminescence Methods and Protocols
This volume presents detailed laboratory protocols regarding the three major route technologies luciferases, efficient optical probes, and applications to visualizing molecular events in living subjects. Bioluminescence: Methods and Protocols, Third
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Sung-Bae Kim Editor
Bioluminescence Methods and Protocols Third Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Bioluminescence Methods and Protocols Third Edition
Edited by
Sung-Bae Kim Research Institute for Environmental Management Technology, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba, Japan
Editor Sung-Bae Kim Research Institute for Environmental Management Technology National Institute of Advanced Industrial Science and Technology (AIST) Tsukuba, Japan
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3811-7 ISBN 978-1-4939-3813-1 (eBook) DOI 10.1007/978-1-4939-3813-1 Library of Congress Control Number: 2016943547 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface Luciferases catalyzing oxidation of luciferins are a nearly ideal reporter for bioanalysis and molecular imaging of intracellular molecular events. Bioluminescent probes fabricated from luciferases generally allow low backgrounds, high signal-to-noise (S/N) ratios, wider dynamic ranges of signals, versatility in the molecular design, and suitability in the imaging of small model animals. Recent studies on bioluminescence-based technologies may be categorized into three major routes: (i) establishment of new luciferases and synthesis of novel luciferins; (ii) fabrication of bioluminescent probes with the luciferases and luciferins; and (iii) practical application of the probes to animal imaging and instrumentations. These three categories are closely correlated in the technical progression: e.g., if we establish de novo luciferases with exce
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