Biosynthesis of Silver Nanoparticles by Methylophilus quaylei , Characterization and Its Impact on Established Biofilms
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Biosynthesis of Silver Nanoparticles by Methylophilus quaylei, Characterization and Its Impact on Established Biofilms Abir M. H. A. Mohamed 1,2 & Vladimir V. Sorokin 3 & Dmitry A. Skladnev 3 & Natalia V. Shevlyagina 4 & Vladimir G. Zhukhovitsky 4 & Anna B. Pshenichnikova 1
# Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract In this study, Methylophilus quaylei proved to possess the ability to reduce AgNO3 and produce silver nanoparticles (AgNPs) under specific conditions via bacterial cells and cell-free supernatant (CFS). The obtained AgNPs via bacterial cells were examined by the transmission of electron microscopy (TEM) consistent with X-ray microanalysis and for AgNPs via CFS by visible (UV–Vis) absorption spectroscopy, scanning electron microscopy (SEM), and dynamic light scattering (DLS). The purpose of this study was to evaluate the antibiofilm effect of AgNPs. The effectiveness of the minimum inhibitory concentration (MIC) of AgNPs in supplementation with CFS was determined on established biofilms on polypropylene by the number of colony forming unit (CFU). These nanoparticles exhibit an antibiofilm effect. CFS was tested individually and in supplementation with the MIC AgNPs obtained on established biofilms of M. quaylei MT and M. quaylei SM. The CFS individually decreased established biofilm for both strains. Further, the obtained MIC of AgNPs in combination with CFS exhibited significant antibiofilm effect against established biofilms for both strains by the destruction of biofilm architecture and detecting cell morphological abnormalities that were observed by SEM. These results showed that the obtained AgNPs had a strong antibiofilm effect against the examined strains. Keywords Methylotrophic bacterium Methylophilus quaylei . Silver nanoparticles . Biosynthesis . Antibiofilm effect
1 Introduction Metal nanoparticles have been widely used due to their enormous applications in different areas, such as medicine, pharmacology, photoimaging and photothermal therapy, electronics, catalysis, optics,. and other various obtaining methods like
* Abir M. H. A. Mohamed [email protected] 1
Department of Biotechnology and Industrial Pharmacy, M. V. Lomonosov Institute of Fine Chemical Technologies, MIREA-Russian Technological University, Moscow, Russian Federation 119571
2
Agricultural Microbiology Department, Faculty of Agriculture, Fayoum University, 63514, Faiyum, Egypt
3
Federal State Institution, Federal Research Centre “Fundamentals of Biotechnology” of the Russian Academy of Sciences, Moscow, Russian Federation
4
Gamaleya Federal Research Centre for Microbiology and Immunology of the Ministry of Public Health, Moscow, Russian Federation
physical, chemical, and biological interactions [1–4]. Silver nanoparticles have been of great scientific interest. They possess antiviral, bactericidal, and fungicidal activities, as well as unique physicochemical properties [5–7]. The wide range of antimicrobial action of silver nanoparticles makes it possible to consider them as a
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