Characterization and antibacterial efficiency of silver nanoparticles biosynthesized by using green algae Enteromorpha i
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ORIGINAL ARTICLE
Characterization and antibacterial efficiency of silver nanoparticles biosynthesized by using green algae Enteromorpha intestinalis Ali Mahdi Haglan1 · Heba S. Abbas3 · Cengiz Akköz1 · Serdar Karakurt2 · Baran Aşikkutlu1 · Erdoğan Güneş1 Received: 7 July 2019 / Accepted: 9 June 2020 © Islamic Azad University 2020
Abstract An ecofriendly and efficient procedure for the synthesis of silver nanoparticles (AgNPs) was performed using aqueous extract of Enteromorpha intestinalis as reducing, capping, and stabilizing agents. AgNPs were characterized by UV spectroscopy, Fourier transform infrared (FTIR), high-resolution transmission electron microscope (HRTEM), and EDX. Moreover, the optimum conditions for the green synthesis were studied and the antimicrobial activity were estimated by agar well diffusion and broth dilution methods. UV vis spectroscopy confirmed the formation of AgNPs due to their surface plasmon resonance. The optimum conditions for the biosynthesis of AgNPs were using 2.5 g or 5 g/100 ml (w/v) of algal biomasses for extraction, and 1 mM of silver ions within 72 h. Transmission electron micrographs showed that AgNPs were spherical in shape and with a mean average size of 9.17 ± 3.2 nm. FTIR revealed the that protein and polysaccharides are responsible for AgNPs synthesis. Additionally, AgNPs exhibited antimicrobial activity against all tested microorganisms. The minimum Inhibitory concentration for all bacteria and Candida albicans ATCC26555 was 6.25 µg/ml except Klebsiella Pneumoniae ATCC70603 and Staphylococcus aureus ATCC4330 was 12.5 µg/ml. Further studies will be recommended to estimate the cytotoxicity of these AgNPs on the human cell line. The future use of algae as green nanofactory will be important in medical applications.
* Heba S. Abbas [email protected] 1
Department of Biology, Faculty of Science, Selçuk University, 42075 Konya, Turkey
2
Department of Biochemistry, Faculty of Science, Selçuk University, 42075 Konya, Turkey
3
Microbiology Department, National Organization for Drug Control and Research, Giza, Egypt
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Vol.:(0123456789)
International Nano Letters
Graphic abstract
13
International Nano Letters
Keywords Enteromorpha intestinalis · Silver nanoparticles · Antimicrobial activity
Introduction There is a tremendous interest in biomedical estimation and revaluation of metallic nanoparticles derived from noble metals because of their specific and unique biological, chemical, and physical features [1]. Researchers explored the use of silver nanoparticles (AgNPs) as a metal nanoparticle for diverse biomedical applications. AgNPs have known their interesting characteristics for progress novel antimicrobial agents or drug delivery preparations, detection, analysis of biomaterials, medical instruments coatings, and improved therapeutic alternatives [2]. Nowadays, there is a growing urgency to advance ecofriendly nanoparticles synthesis routes. The ecofriendly biological synthesis method of nanoparticles using microorganisms, plants, and alga
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