Biosynthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Passiflora subpeltata : Characterization and Antibacteria
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ORIGINAL PAPER
Biosynthesis of Zinc Oxide Nanoparticles Using Leaf Extract of Passiflora subpeltata: Characterization and Antibacterial Activity Against Escherichia coli Isolated from Poultry Faeces Udayashankar Arakere Chunchegowda1 • Ashwini Bagepalli Shivaram2 • Murali Mahadevamurthy3 Lakshmeesha Thimappa Ramachndrappa4 • Sreelatha Gopalakrishna Lalitha5 • Hemanth Kumar Naguvanahalli Krishnappa6 • Satish Anandan7 • Brijesh Singh Sudarshana1 • Eswaraiah Ganadhal Chanappa8 • Niranjana Siddapura Ramachandrappa1
•
Received: 30 May 2020 / Accepted: 26 October 2020 Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract The current study was undertaken to investigate the antibacterial (against molecular characterized E. coli isolated from poultry faeces) potential of biosynthesized zinc oxide nanoparticles (ZnO-NPs) from Passiflora subpeltata Ortega aqueous leaf extract. The biosynthesized nanoparticles were subjected to physico-chemical characterization to study shape, size and purity by UV–Vis spectroscopy, X-Ray diffraction (XRD), Fourier Transform Infrared Spectroscopy (FT-IR), Scanning Electron Microscopy (SEM), Energy Dispersive Spectroscopy (EDS) and Transmission Electron Microscopy (TEM). The molecular identification of isolated E. coli from faeces samples was carried out by using 16–23s rRNA primers. The results of the physico-chemical characterization revealed that the biosynthesized nanoparticles were of 93.7% purity with an average size between 45 and 50 nm. The ZnO-NPs offered significant inhibition against the isolated Gram-negative E. coli with MIC at 62.5 lg mL-1 concentration. The antibacterial potential of ZnO NPs against E. coli has also been investigated by the cell viability test, and further the effects of ZnO NPs on bacterial morphological structures was analysed by SEM and TEM. Keywords E. coli Poultry Cell viability TEM Passiflora subpeltata Zinc oxide nanoparticles
Arakere Chunchegowda Udayashankar and Bagepalli Shivaram Ashwini have contributed equally to this work. 5
Department of Crop Protection, ICAR-Central Tuber Crops Research Institute, Sreekariyam, Thiruvananthapuram 695 017, India
6
Department of Studies in Botany, University of Mysore, Manasagangotri, Mysuru, Karnataka 570 006, India
7
Department of Studies in Food Science and Nutrition, University of Mysore, Manasagangotri, Mysuru, Karnataka 570 006, India
8
Government Science College, NT Road, Bengaluru 560001, India
& Niranjana Siddapura Ramachandrappa [email protected] 1
Department of Studies in Biotechnology, University of Mysore, Manasagangotri, Mysuru, Karnataka 570 006, India
2
Department of Microbiology, Sri Siddhartha Medical College, Tumakuru, Karnataka 572107, India
3
Applied Plant Pathology Laboratory, Department of Studies in Botany, University of Mysore, Manasagangotri, Mysuru, Karnataka 570 006, India
4
Department of Microbiology and Biotechnology, Jnanabharathi Campus, Bangalore University, Bengaluru, Karnataka
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