Brassinosteroids Methods and Protocols

This detailed volume compiles state-of-the-art methodologies for the study of brassinosteroid hormones, contributed by recognized researchers in the field, in order to bring together different experimental and theoretical biology techniques for addressing

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Eugenia Russinova Ana I. Caño-Delgado Editors

Brassinosteroids Methods and Protocols

Methods

in

Molecular Biology

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Brassinosteroids Methods and Protocols

Edited by

Eugenia Russinova VIB-Ghent University, Departments of Plant Systems Biology and Plant Biotechnology and Bioinformatics, Ghent, Belgium

Ana I. Caño-Delgado Research in Argicultural Genomics (CRAG), CSIC-IRTA-UAB-UB, Cerdanyola del Vallès, Barcelona, Spain

Editors Eugenia Russinova VIB-Ghent University Departments of Plant Systems Biology and Plant Biotechnology and Bioinformatics Ghent, Belgium

Ana I. Caño-Delgado Research in Argicultural Genomics (CRAG) CSIC-IRTA-UAB-UB Cerdanyola del Vallès, Barcelona, Spain

ISSN 1064-3745     ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6811-4    ISBN 978-1-4939-6813-8 (eBook) DOI 10.1007/978-1-4939-6813-8 Library of Congress Control Number: 2016961692 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover Illustration: The book cover image illustrates the localization of brassinosteroid receptor BRL3 enriched at stem cell niche of the root meristem in Arabidopsis. Understanding the molecular bases for cell-specific action of brassinosteroid signalling opens new and exciting possibilities for understanding plant growth and adaption to the changing environment. Image taken with permission from ASPB as reported in: Fabregas, N, Li, N, Boeren, S., Nash, T.E., Goshe, M.B., Clouse S.D., de Vries S. and Caño-Delgado, A.I. Specific roles for BRL3 signalosome complex in Arabidopsis root development. Plant Cell, 25(9); 3377-3388 (2013). doi: 10.1105/tpc.113.114462 Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The regist