Cellulases Methods and Protocols

This volume discusses the latest research and techniques used to study novel cellulases. The chapters in this book explore topics such as the role of cellulases in lignocellulose deconstruction for generation of a sugar platform for biofuel; screening for

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Mette Lübeck Editor

Cellulases Methods and Protocols

METHODS

IN

MOLECULAR BIOLOGY

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Cellulases Methods and Protocols

Edited by

Mette Lübeck Section of Sustainable Biotechnology, Department of Chemistry and Bioscience, Aalborg University, Copenhagen, Denmark

Editor Mette Lu¨beck Section of Sustainable Biotechnology Department of Chemistry and Bioscience Aalborg University Copenhagen, Denmark

ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7876-2 ISBN 978-1-4939-7877-9 (eBook) https://doi.org/10.1007/978-1-4939-7877-9 Library of Congress Control Number: 2018941816 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface Cellulases, especially of microbial origin, have applications in various industries including pulp and paper, textile, laundry, food and feed industry, brewing, and agriculture. Cellulases are becoming increasingly important as key enzymes for biomass deconstruction within biorefineries, for the production of fuels and chemicals. Cellulases are thereby of utmost importance in the transition of the society into a more sustainable, biobased economy. Research in discovery, improvement, and applications of novel cellulases is growing rapidly, not only at the university level but also in industry. The aim of this book is to become the definite lab protocol source in this important area. The target audience is biochem