Pseudoxanthomonas humi sp. nov., a bacterium isolated from rhizospheric soil of Fraxinus chinensis in Gyeonggi Province,
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ORIGINAL PAPER
Pseudoxanthomonas humi sp. nov., a bacterium isolated from rhizospheric soil of Fraxinus chinensis in Gyeonggi Province, South Korea Shahina Akter1 · Hae Jo2 · Juan Du1 · KyungHwa Won1 · Chang Shik Yin3 · MooChang Kook4 · Hongshan Yu5 · Han‑Seok Choi6 · Myung‑Kon Kim7 · Tae‑Hoo Yi1,2
Received: 4 July 2015 / Revised: 4 September 2015 / Accepted: 26 September 2015 / Published online: 6 October 2015 © Springer-Verlag Berlin Heidelberg 2015
Abstract A novel bacterial strain THG-MM13T was isolated from rhizospheric soil sample and was characterized by using a polyphasic approach. Cells were Gramreaction-negative, non-motile and rod-shaped. The strain was aerobic, catalase and oxidase positive, and optimum growth temperature and pH were 28 °C and 7.0, respectively. On the basis of 16S rRNA gene sequence analysis, strain THG-MM13T (KM598260) belongs to the genus Pseudoxanthomonas and is most closely related to Pseudoxanthomonas wuyuanensis KCTC 23877T (97.4 %) (JN247803), followed by Pseudoxanthomonas koreensis KCTC 12208T (96.7 %) (AY550263) and Pseudoxanthomonas yeongjuensis KACC 11580T (96.7 %) (DQ438977). The DNA G + C content was 63.7 mol%, Communicated by Erko Stackebrandt. Shahina Akter and Hae Jo have equally contributed to this work. The NCBI GenBank accession number for the 16S rRNA gene sequence of strains THG-MM13T is KM598260. Electronic supplementary material The online version of this article (doi:10.1007/s00203-015-1157-5) contains supplementary material, which is available to authorized users. * Tae‑Hoo Yi [email protected] 1
Department of Oriental Medicinal Material and Processing College of Life Science, Kyung Hee University Global Campus, 1732 Deokyoungdaero, Giheung‑gu, Gyeonggi‑do, Yongin‑si 446‑701, Republic of Korea
2
Graduate School of Biotechnology, Kyung Hee University, Gyeonggi‑do, Republic of Korea
3
Department of Acupuncture Meridian Science Research Center, College of Korean Medicine, Kyung Hee University Global Campus, Seoul, Republic of Korea
and the predominant respiratory quinone was ubiquinone-8. The major polar lipids were diphosphatidylglycerol and phosphatidylethanolamine. The major fatty acids were iso-C15:0 (31.3 %) and iso-C16:0 (19.3 %). The DNA–DNA relatedness value between strain THG-MM13T and P. wuyuanensis KCTC 23877T was below 50 %. The DNA–DNA hybridization result and results of the genotypic analysis in combination with chemotaxonomic and physiological data demonstrated that strain THG-MM13T represented a novel species within the genus Pseudoxanthomonas, for which the name Pseudoxanthomonas humi is proposed. The type strain is THG-MM13T (=KACC 18280T = CCTCC AB 2015122T). Keywords Pseudoxanthomonas humi · Gram-reactionnegative · 16S rRNA · Ubiquinone-8
Introduction The genus Pseudoxanthomonas, belonging to the family Xanthomonadaceae, order Xanthomonadales, class Gammaproteobacteria, phylum Proteobacteria (Garrity and 4
Department of Marine Biotechnology, Anyang University, Incheon 417‑833, Republic of Korea
5
College of Bio and F
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