Cilia Methods and Protocols
This volume is the result of an explosion of molecular-based research on Cilia, which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/proteomics. The chapters in this book cover topics such as: high reso
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Peter Satir Søren Tvorup Christensen Editors
Cilia Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Cilia Methods and Protocols
Edited by
Peter Satir Albert Einstein College of Medicine, Yeshiva University, Bronx, NY, USA
Søren Tvorup Christensen Department of Biology, University of Copenhagen, Copenhagen, Denmark
Editors Peter Satir Albert Einstein College of Medicine Yeshiva University Bronx, NY, USA
Søren Tvorup Christensen Department of Biology University of Copenhagen Copenhagen, Denmark
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-3787-5 ISBN 978-1-4939-3789-9 (eBook) DOI 10.1007/978-1-4939-3789-9 Library of Congress Control Number: 2016943552 © Springer Science+Business Media New York 2016 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC New York
Preface This volume is the result of an explosion of molecular-based research on cilia which began with the discovery of the universality of intraflagellar transport (IFT) and ciliary genomics/ proteomics at the turn of the millennium and attracted new interest and new investigators into the field. The cilium is a cell organelle with nanometer substructure which can be studied with techniques at the cutting edge of molecular biology. We invited expert contributors associated with these techniques, their sometimes specialized instrumentation and their implications for ciliary biology, to join us in writing chapters for the volume. Unlike previous Methods volumes, this one is broadly based, covering motile, sensory, and primary cilia and it should be attractive to anyone interested in entering the field of ciliary biology using model orga
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