Clathrin-Mediated Endocytosis Methods and Protocols
This book presents a methodological introduction on clathrin-mediated endocytosis. Chapters guide readers through a number of model systems, purifications, reconstitutions, analysis techniques and systemic alterations of membrane function, which has been
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Laura E. Swan Editor
ClathrinMediated Endoytosis Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Clathrin-Mediated Endoytosis Methods and Protocols
Edited by
Laura E. Swan Department of Cellular & Molecular Physiology, University of Liverpool, Institute of Translational Medicine, Liverpool, UK
Editor Laura E. Swan Department of Cellular & Molecular Physiology University of Liverpool Institute of Translational Medicine Liverpool, UK
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8717-7 ISBN 978-1-4939-8719-1 (eBook) https://doi.org/10.1007/978-1-4939-8719-1 Library of Congress Control Number: 2018950658 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The regulated retrieval of plasma membrane and its cargo proteins is fundamental to a cell’s interaction with its environment. In a membrane bristling with transmembrane receptors, at any given moment some of them signaling, some of them silent—the ability to selectively isolate, concentrate, and retrieve specific cargoes can dynamically and locally regulate cell signaling, cell adhesion, and membrane properties. One of the best-understood modes of regulated membrane retrieval is clathrin-mediated endocytosis (CME): the assemblage of a macromolecular scaffold of membrane-associated adaptor proteins which are progressively “caged” by clathrin triskelia, bud off the plasma
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