Genetic diversity of N-fixing and plant growth-promoting bacterial community in different sugarcane genotypes, associati
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ORIGINAL PAPER
Genetic diversity of N‑fixing and plant growth‑promoting bacterial community in different sugarcane genotypes, association habitat and phenological phase of the crop Danubia Ramos Moreira de Lima1 · Isaneli Batista dos Santos2 · João Tiago Correia Oliveira3 · Diogo Paes da Costa4 · João Victor Jansen de Queiroz1 · Emiliana Manesco Romagnoli2 · Fernando Dini Andreote2 · Fernando José Freire1 · Júlia Kuklinsky‑Sobral4 Received: 6 July 2020 / Revised: 15 October 2020 / Accepted: 19 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract This study aimed to evaluate the genetic diversity of bacterial community associated to different sugarcane genotypes, association habitat and phenological phase of the culture, as well as to isolate, to identify and to characterize your potential for plant growth-promoting. Root and rhizospheric soil samples from RB 92579 and RB 867515 varieties were collected at 120 and 300 days after regrowth (DAR). The diversity of bacterial was evaluated through of the 16S rRNA and nifH genes. We found greater genetic diversity in the root endophytic habitat at 120 DAR. We identify the genera Burkholderia sp., Pantoea sp., Erwinia sp., Stenotrophomonas sp., Enterobacter sp. and Pseudomonas sp. The genera Bacillus sp. and Dyella sp. were only identified in the variety RB 92579. We found indices above 50% for biological nitrogen fixation, production of indole acetic acid and phosphate solubilization, showing that the use of these bacteria in biotechnological products is very promising. Keywords BOX-PCR · DGGE · Biological N fixation · Production of indole acetic acid · Phosphate solubilization
Introduction Sugarcane nitrogen (N) demand in the second crop cycle (ratoon-cane) increases by an average of 50% in relation to the first crop cycle (plant-cane) (Sattolo et al. 2017). The Communicated by Erko Stackebrandt. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02103-7) contains supplementary material, which is available to authorized users. * Fernando José Freire [email protected] 1
Departamento de Agronomia, Universidade Federal Rural de Pernambuco, Recife 52171‑900, Brasil
2
Departamento de Microbiologia Agrícola, Escola Superior de Agricultura “Luiz de Queiroz”, Universidade de São Paulo, Piracicaba 13418‑900, Brasil
3
Instituto de Estudos do Trópico Úmido, Universidade Federal do Sul e Sudeste do Pará, Marabá 68507‑590, Brasil
4
Laboratório de Biotecnologia e Genética Microbiana, Universidade Federal do Agreste de Pernambuco, Garanhuns 55292‑270, Brasil
use of chemical N fertilizer to meet this elevated demand increases the probability of N loss in the soil–plant-atmosphere system, increasing its environmental impact (Kyllmar et al. 2014) and creating a need for studies examining alternatives that may reduce the use of N fertilizers, such as biological N fixation (BNF). BNF can be performed in sugarcane by several bacterial genera, such as: Beijerink
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