Cyclooxygenases Methods and Protocols
Since the discovery of the pharmacological and toxicological importance of inhibiting the cyclooxygenase (COX) enzymes by non-steroidal anti-inflammatory drugs (NSAIDs), much research has gone into the development of methods to study the biological functi
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Cyclooxygenases Methods and Protocols
Edited by
Samir S. Ayoub, Roderick J. Flower, and Michael P. Seed Centre for Biochemical Pharmacology, Experimental Pathology Group, St. Bart’s and the London School of Medicine and Dentistry, William Harvey Research Institute, Queen Mary University of London, London, UK
Editors Samir S. Ayoub, Ph.D. Centre for Biochemical Pharmacology, Experimental Pathology Group St. Bart’s and the London School of Medicine and Dentistry William Harvey Research Institute Queen Mary University of London London, UK [email protected]
Roderick J. Flower, Ph.D. Centre for Biochemical Pharmacology St. Bart’s and the London School of Medicine and Dentistry William Harvey Research Institute Queen Mary University of London London, UK [email protected]
Michael P. Seed, Ph.D. Centre for Biochemical Pharmacology, Experimental Pathology Group St. Bart’s and the London School of Medicine and Dentistry William Harvey Research Institute Queen Mary University of London London, UK [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-58829-953-6 e-ISBN 978-1-59745-364-6 DOI 10.1007/978-1-59745-364-6 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010923614 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is a part of Springer Science+Business Media (www.springer.com)
Preface The nonsteroidal anti-inflammatory drugs (NSAIDs) consist of more than 40 compounds, which are the most widely used group of medicines worldwide for the treatment of inflammation, pain, fever, and for the prevention of thrombosis. It was through the pivotal work of the late Professor Sir John Vane in the early 1970s that it was realized t
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