Cytoskeleton Methods and Protocols

In the ten years since the publication of the first edition, great advances in fluorescent labeling, optics, and sample preparation have significantly improved the imaging capability of microscopy, allowing for a continual refinement of our understanding

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Molecular Biology™

Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK



For other titles published in this series, go to www.springer.com/series/7651

Cytoskeleton Methods and Protocols Edited by

Ray H. Gavin Brooklyn College of The City University of New York, Brooklyn, NY, USA

Editor R.H. Gavin Brooklyn College of The City University of New York Brooklyn, NY USA [email protected]

ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60761-375-6 e-ISBN 978-1-60761-376-3 DOI 10.1007/978-1-60761-376-3 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2009930645 © Humana Press, a part of Springer Science+Business Media, LLC 2009 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or ­dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, ­neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Cover illustration: The cover illustration shows a field of dividing and non-dividing Xenopus tissue culture cells stained for microtubules (green), DNA (red), and a soluble protein that accumulates in nuclei during interphase and distributes throughout the cytoplasm during mitosis (blue). In the mitotic cell near the center of the image, the highly condensed chromosomes are aligned on the mitotic spindle, the elaborate macromolecular machine that is responsible for moving sister chromatids apart during cell division. Prominent in the two cells just below it are the remnants of the mitotic spindle (named the midbody) that persists as the daughter cells finish cytokinesis and enter the next interphase. Microtubules in the non-dividing cells at the periphery of the image are longer and are arranged in a radial pattern around a single centrosome in each cell (not shown; please note that the nuclei of these cells are also not visible in this image). Photo courtesy Christiane Wiese and Adam Steinberg Printed on acid-free paper Humana is part of Springer Science+Business Media (www.springer.com)

Preface In the 10 years since the publication of the first edition of Cytoskeleton Methods and ­Protocols, advances in