Ebolaviruses Methods and Protocols
This vital book collects key methods that have supported advancements in the field of ebolavirus molecular biology given the pressing need for the advancement of techniques for diagnostics, the development of vaccines and antivirals, and for furthering ou
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Thomas Hoenen Allison Groseth Editors
Ebolaviruses Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Ebolaviruses Methods and Protocols
Edited by
Thomas Hoenen • Allison Groseth Friedrich-Loeffler-Institut, Greifswald - Insel Riems, Germany
Editors Thomas Hoenen Friedrich-Loeffler-Institut Greifswald - Insel Riems Germany
Allison Groseth Friedrich-Loeffler-Institut Greifswald - Insel Riems Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7115-2 ISBN 978-1-4939-7116-9 (eBook) DOI 10.1007/978-1-4939-7116-9 Library of Congress Control Number: 2017940477 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: Electron micrograph of an ebolavirus-infected cell, provided by Prof. Takeshi Noda (Kyoto University, Japan). Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Dedication The editors wish to dedicate this book to Michael P. Kiley (1942–2004), whose own groundbreaking work during the early years taught us much of what we know today about the molecular biology of ebolaviruses. We hope he would have been proud of all the discoveries for which his own work paved the way.
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Preface While filoviruses as a group were first discovered 50 years ago, and ebolaviruses have been known since 1976, many of these viruses’ secrets remained hidden until the use of molecular biological techniques became widespread at the end of the 1980s. Since then, advances, first in sequencing and clon
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