Electroanalytical application of thiol-grafted laponite to the sensitive quantification of ciprofloxacin antibiotic
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RESEARCH ARTICLE
Electroanalytical application of thiol‑grafted laponite to the sensitive quantification of ciprofloxacin antibiotic Kevin Yemélé Tajeu1 · Delmas Vidal Tabe Ebunang1 · Ranil Clément Temgoua Tonleu1 · Sherman Lesly Zambou Jiokeng1 · Ervice Ymele1 · Ignas Kenfack Tonle1 Received: 10 June 2020 / Accepted: 9 November 2020 © Springer Nature B.V. 2020
Abstract In this study, a sensitive and selective voltammetric sensor was proposed for the detection of ciprofloxacin (CFX) fluoroquinolone-based antibiotic, using a glassy carbon electrode (GCE) modified by a thin film of thiol functionalized laponite (LaSH). The employed organolaponite was prepared by surface grafting of 3-mercaptopropyltrimethoxysilane (MPTMS), then characterized by XRD, FTIR spectroscopy, thermal analysis and CHS elemental analysis. The electrochemical behavior of CFX was investigated on the thin film GCE/LaSH by cyclic voltammetry, which showed a single irreversible oxidation peak at 1.3 V (vs. Ag/AgCl) in a Britton-Robinson buffer solution at pH 2. The CFX current response on the organoclay modified electrode was significantly improved, due to favorable interactions between thiol functional groups and CFX. Differential pulse stripping voltammetry was then applied for quantitative determinations, and key parameters such as pH of detection medium, accumulation time, agitation and CFX concentration were optimized. In optimum experimental conditions, a linear relationship between the peak current density and CFX concentration was observed in the range 10.0–110.0 µM, with a detection limit of 2.69 × 10–7 M (S/N = 3). Kinetic and chronocoulometric studies were also performed to characterize the diffusion of CFX at the modified organoclay electrode. The effect of some important potential interfering compounds on the stripping signal of CFX was also examined, followed by the application of the developed sensor in the determination of CFX in a pharmaceutical formulation using the standard addition method.
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s10800-020-01508-y) contains supplementary material, which is available to authorized users. * Ignas Kenfack Tonle ignas.tonle@univ‑dschang.org 1
Electrochemistry and Chemistry of Materials, Department of Chemistry, Faculty of Science, University of Dschang, P.O. Box 67, Dschang, Cameroon
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Vol.:(0123456789)
Journal of Applied Electrochemistry
Graphic abstract Laponite clay mineral functionalised with thiol groups from MPTMS, exhibited more affinity towards Ciprofloxacin antibiotic, allowing its voltammetric detection in a commercial pharmaceutical sample
Keywords Organoclay · Grafting · Ciprofloxacin · Modified electrode · Electroanalysis
1 Introduction Fluoroquinolones have been recognized as an important group of synthetic broad-spectrum antibiotics since their first introduction in the early 1980s [1]. Ciprofloxacin (CFX) [1-cyclopropyl-6-fluoro-1,4-dihydro-4-oxo-7 (piperazinyl) quinolone-3-carboxylic acid], a second-genera
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