ELISA Methods and Protocols
This volume is a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA), used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany and qua
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Robert Hnasko Editor
ELISA
Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
ELISA Methods and Protocols
Edited by
Robert Hnasko Agricultural Research Service (ARS), U.S. Department of Agriculture, Albany, CA, USA
Editor Robert Hnasko Agricultural Research Service (ARS) U.S. Department of Agriculture Albany, CA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-2741-8 ISBN 978-1-4939-2742-5 (eBook) DOI 10.1007/978-1-4939-2742-5 Library of Congress Control Number: 2015943593 Springer New York Heidelberg Dordrecht London © Springer Science+Business Media New York 2015 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover illustration: Rendered three dimensional image of immunoglobulin (IgG1) protein as space filled and ribbon structure. Coordinates for IgG1 obtained from pdb1igy.pdb (Harris LJ, et al. J. Mol. Biol. 1998;275:861–872). Image by Robert Hnasko. Printed on acid-free paper Humana Press is a brand of Springer Springer Science+Business Media LLC New York is part of Springer Science+Business Media (www.springer.com)
Preface This book is intended as a practical biochemical guide to the Enzyme-Linked Immunosorbent Assay (ELISA) used to detect a target substance in a liquid sample. The ELISA is an important and widely used diagnostic tool in medicine, animal health, botany, and quality assurance processes in food and beverage production. The adoption of the ELISA by these industries is in part driven by the sensitivity and selectivity by which the assay can detect a desired target analyte along with it potential for standardization, automation, and economy of cost. The component parts of the ELISA are well suited for commercialization and adaptable for target detection using a diverse range of platforms. The antibody is central
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