Erythropoiesis Methods and Protocols
This detailed volume provides thorough protocols describing how to use genetics to study mouse and zebrafish erythropoiesis in whole animal models and for genetically manipulating cultured mouse and human erythroid cells. Protocols include strategies to a
- PDF / 7,753,692 Bytes
- 302 Pages / 504.63 x 737.01 pts Page_size
- 10 Downloads / 174 Views
Joyce A. Lloyd Editor
Erythropoiesis Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Erythropoiesis Methods and Protocols
Edited by
Joyce A. Lloyd Department of Human & Molecular Genetics, Virginia Commonwealth University, Richmond, VA, USA Massey Cancer Center, Virginia Commonwealth University, Richmond, VA, USA
Editor Joyce A. Lloyd Department of Human & Molecular Genetics Virginia Commonwealth University Richmond, VA, USA Massey Cancer Center Virginia Commonwealth University Richmond, VA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7427-6 ISBN 978-1-4939-7428-3 (eBook) DOI 10.1007/978-1-4939-7428-3 Library of Congress Control Number: 2017956114 © Springer Science+Business Media LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The aim of this volume is to provide a definitive source for molecular and cellular biology protocols to study erythropoiesis. The target audience is molecular biologists, biochemists, and geneticists who are studying erythropoiesis. The methods span the mouse, human, and zebrafish systems, because comparing model systems has historically provided a comprehensive approach to erythropoiesis. Red blood cells are the stuff of life, so it is natural that erythropoiesis has been an active area of inquiry for centuries. Yet questions remain, and many of these have clinical relevance to anemia conditions. How does hemoglobin switching occur, and can the
Data Loading...
