Exosomes and Microvesicles Methods and Protocols
This volume covers methods for the analysis of extracellular vesicles (EV) that can be applied to isolated EVs from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analys
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Andrew F. Hill Editor
Exosomes and Microvesicles Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Exosomes and Microvesicles Methods and Protocols
Edited by
Andrew F. Hill Department of Biochemistry and Genetics, La Trobe Institute for Molecular Science, La Trobe University, Bundoora, VIC, Australia
Editor Andrew F. Hill Department of Biochemistry and Genetics La Trobe Institute for Molecular Science La Trobe University Bundoora, VIC, Australia
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6726-1 ISBN 978-1-4939-6728-5 (eBook) DOI 10.1007/978-1-4939-6728-5 Library of Congress Control Number: 9781493967261 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Exosomes and Microvesicles: Methods and Protocols brings together a collection of methods for studying extracellular vesicles (EV). There has been significant growth in the field of EV research over the last decade as we understand more about the role of exosomes, microvesicles, and other EVs in many facets of cellular biology. This has been brought about with the emerging role of EVs in cell-cell communication and their potential as sources of disease biomarkers and a delivery agent for therapeutics. The protocols in this volume of Methods in Molecular Biology cover methods for the analysis of EVs which can be applied to those isolated from a wide variety of sources. This includes the use of electron microscopy, tunable resistance pulse sensing, and nanoparticle tracking analysis. Furthermore, analysis
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