Expressions of Pathogenesis related 1 ( PR1 ) Gene in Solanum lycopersicum and Influence of Salicylic Acid Exposures on
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IOCHEMISTRY, BIOPHYSICS, AND MOLECULAR BIOLOGY
Expressions of Pathogenesis related 1 (PR1) Gene in Solanum lycopersicum and Influence of Salicylic Acid Exposures on Host-Meloidogyne incognita Interactions Refik Bozbuga Presented by Academician S.N. Kochetkov Received June 19, 2020; revised June 19, 2020; accepted June 19, 2020
Abstract– Salicylic acid (SA) induces the mechanism of the plant defence and plays a role in plant -pathogen interactions. Following the nematode infection, effects of SA treatment on plant weights and the expression of pathogen related gene have not been fully understood. The present study was aimed to determine the effects of SA treatment on the expression Pathogenesis related 1 gene (PR1 gene) and alteration on plant parameters in tomatoes (Solanum lycopersicum). Plant seedlings were dripped within the SA solution. The expression of PR1 gene achieved using RT-PCR at 1, 3, 7, 14, 21-days post infection (dpi) with Meloidogyne incognita. Upregulation of PR1 gene was determined in early (1 dpi) and late (21 dpi). SA treatment and nematode infection altered plant parameters. SA treatment increased the plant defence mechanisms in tomato against Meloidogyne incognita. Keywords: Pathogenesis related 1 gene, nematode, tomato, Meloidogyne incognita, salicylic acid, gene expression DOI: 10.1134/S1607672920050038
INTRODUCTION Parasitic nematodes associated with plantae are important pests in globe that cause extensive damage to numerous cultivated crops. Meloidogyne species are obligate parasites infecting most plant species and causing galls on roots of many plants [1]. This nematode causes modification of cell wall molecular architecture in nematode feeding sites [2]. Some tomato genotypes have resistance gene (Mi gene) against M. incognita [3]. During the infection, pathogens can be recognised by greatly conserved pathogen-associated molecular patterns of host plant, and then host’s immunity inhibits the growth of infecting agent [4]. PR proteins are activated in response to pathogens attack [5]. As a significant hormone, SA participates the plant defence regulations, plant growth and plant development [6]. Transcription co-activator, NPR1 is a fundamental regulator of the plant defence response [7]. The alterations of plant gene expressions are linked with nematode parasitism. Meloidogyne incognita was selected as a major nematode species with wide host range including tomato
Biological Control Research Institute, Nematology Lab, Yuregir, Adana, 01321 Turkey e-mail: [email protected]
plants. Effects of salicylic acid (SA) on tomato Solanum lycopersicum defence against M. incognita including expression of related Pathogenesis related (PR) gene have not been fully understood. In the present work different exposure time of SA as an elicitor of resistance in tomato infection by M. incognita have been tested and PR gene expression provoked by nematode infection using qRT-PCR have been estimated. MATERIAL AND METHODS Nematode inoculum and plant culture. Pure population of Meloidogyne incognita w
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