Fibrosis Methods and Protocols
This volume describes state-of-the-art protocols that serve as “recipes” for scientists concentrating on fibrosis research. This book is divided into four sections. Part I focuses on animal models of fibrosis and covers topics such as mimicking fibrosis i
- PDF / 27,026,089 Bytes
- 520 Pages / 504.63 x 737.01 pts Page_size
- 79 Downloads / 310 Views
Laure Rittié Editor
Fibrosis Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Fibrosis Methods and Protocols
Edited by
Laure Rittié Department of Dermatology, University of Michigan Medical School, Ann Arbor, MI, USA; Dermatology Therapeutic Area, GlaxoSmithKline, Collegeville, PA, USA
Editor Laure Rittié Department of Dermatology University of Michigan Medical School Ann Arbor, MI, USA Dermatology Therapeutic Area GlaxoSmithKline Collegeville, PA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7112-1 ISBN 978-1-4939-7113-8 (eBook) DOI 10.1007/978-1-4939-7113-8 Library of Congress Control Number: 2017940794 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The term “fibrosis” designates the formation of excess fibrous connective tissue that can affect a plethora of tissues and organs. Extracellular matrix (ECM) deposition is typically a normal reaction to injury that allows tissue repair and restoration of tissue strength. However, when the response turns awry, ECM deposition becomes pathogenic and results in thickened extracellular environment that hampers tissue properties and may lead to organ failure. Fibrosis’ prevalence within so many organs makes it quite complex to study. Researchers thereby rely on multiple in vitro, ex vivo, and in vivo models aimed at recapitulating one of several aspects of the fibrotic reaction. Fibrosis:
