Germline Development Methods and Protocols
The germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation, making these cells amenable to in-depth genomic and mechanistic studies. In Germline Development: Methods and Protocols, expert research
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Germline Development Methods and Protocols Edited by
Wai-Yee Chan School of Biomedical Sciences, The Chinese University of Hong Kong, Shatin, N.T., Hong Kong SAR, China
Le Ann Blomberg ARS-USDA, Animal Biosciences & Biotechnology Lab, Beltsville, MD, USA
Editors Wai-Yee Chan, Ph.D. School of Biomedical Sciences The Chinese University of Hong Kong Shatin, N.T., Hong Kong SAR, China [email protected]
Le Ann Blomberg, Ph.D. ARS-USDA Animal Biosciences & Biotechnology Lab Beltsville, MD, USA [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-435-3 e-ISBN 978-1-61779-436-0 DOI 10.1007/978-1-61779-436-0 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011941357 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Proper development of the germ cells (i.e., germline) is essential for the propagation of mammalian species. In spite of recent advances in the biological sciences, our knowledge of the mechanisms that regulate germline development is still rather limited. This volume aims to provide a comprehensive collection of techniques that can be applied to the study of both the male and female germline. The germline is unique in mammals as it is the only cell lineage that undergoes mitosis, meiosis, and differentiation. Furthermore, the lineage comprises an array of developmentally distinct stages from the germinal stem cell to the terminally differentiated sperm or oocyte. Fortunately, cells at unique stages of development can be isolated as relatively pure populations. This feature makes these cells amenable to in-depth genomic and mechanistic studies. Knowledge of the biology of cells of either the male or female lineage will provide us with a better appreciation of the cellular mechanisms modulating the germline developmental processes. In addition, understanding how a germinal stem cell maintains stemness versus the induction of its differentiation will provide us insight on how to direct the pathway of differentiation
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