Synapse Development Methods and Protocols
This detailed volume collects protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. Contributed by experts and pioneers in their respective techniques, the book covers synapses in the br
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Alexandros Poulopoulos Editor
Synapse Development Methods and Protocols
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Synapse Development Methods and Protocols
Edited by
Alexandros Poulopoulos Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, MA, USA
Editor Alexandros Poulopoulos Department of Stem Cell and Regenerative Biology Harvard University Cambridge, MA, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6686-8 ISBN 978-1-4939-6688-2 (eBook) DOI 10.1007/978-1-4939-6688-2 Library of Congress Control Number: 2016956874 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. Cover Illustration: Cover image design by Massimiliano Stagi Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface This book is a collection of protocols for experimentation into how neurons connect to produce the extraordinary functionalities of the nervous system. The complexity underlying the development of synapses allows and warrants an assortment of approaches to probe the many layers of structure, mechanism, and emergent function. Appropriately, neuroscience is populated by researchers across disciplines, and with them comes a range of technologies and methodologies that make the neuroscience toolkit one of characteristic wealth. The experimental diversity converging onto the synapse has made it one of the most intensely studied and intricately described biological systems. Observing in real-time how a single protein flips between conformational states to regulate ion flows, following how thin cellular protrusions traverse the
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