Handbook of ELISPOT Methods and Protocols
This third edition volume expands on the previous editions with new protocols and up-to-date techniques to help researchers excel in this field. The chapters in this book cover topics such as ELISpot’s sibling (FluoroSpot); ELISpot for influenza, Dengue v
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Alexander E. Kalyuzhny Editor
Handbook of ELISPOT Methods and Protocols Third Edition
Methods
in
M o l e c u l a r B i o lo g y
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Handbook of ELISPOT Methods and Protocols Third Edition
Edited by
Alexander E. Kalyuzhny Bio-Techne, Inc., Minneapolis, MN, USA
Editor Alexander E. Kalyuzhny Bio-Techne, Inc. Minneapolis, MN, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-8566-1 ISBN 978-1-4939-8567-8 (eBook) https://doi.org/10.1007/978-1-4939-8567-8 Library of Congress Control Number: 2018946639 © Springer Science+Business Media, LLC, part of Springer Nature 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Despite technological advancements and the availability of ultrasensitive analytical methods, ELISpot assay, which is technically quite simple, is firmly holding its ground for studying the cytokine-secreting activity of immune system cells. Furthermore, this assay is gaining a reputation as a robust and reliable research and diagnostic tool. To refresh readers’ memories, it is worth mentioning that the first edition of the Handbook of ELISpot was released in 2005. Due to a strong interest from the scientific community, it was followed by a second edition published in 2012. We were excited to learn that our second edition also received strong positive feedback, which prompted us to continue with a third edition. It appears that ELISpot remains to be a very dynamic techniq
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