Flavihumibacter soli sp. nov., Isolated from Soil
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Flavihumibacter soli sp. nov., Isolated from Soil Ye Lin Seo1 · Jaejoon Jung1 · Shehzad Abid Khan1 · Kyung Hyun Kim1 · Che Ok Jeon1 Received: 18 March 2020 / Accepted: 18 June 2020 © Springer Science+Business Media, LLC, part of Springer Nature 2020
Abstract A novel Gram-stain-positive, strictly aerobic, non-motile, rod-shaped bacterium, designated strain R14T was isolated from steep grove soil and taxonomically characterized using a polyphasic approach. Cells showed oxidase-negative and catalasepositive activities and grew at 25–37 °C (optimum, 30 °C), pH 5.0–8.0 (optimum, pH 6.5), and in the presence of 0–0.5% (w/v) NaCl (optimum, 0%). Phylogenetic trees based on 16S rRNA gene sequences revealed that strain R14T formed a phylogenetic lineage with Flavihumibacter genus members within the family Chitinophagaceae. Comparison of 16S rRNA gene sequences showed that strain R14T shared highest similarities with Flavihumibacter solisilvae 3-3T (97.5%), Flavihumibacter petaseus NBRC 106054T (96.7%), and Flavihumibacter stibioxidans YS-17T (96.1%). The G+C content of strain R14T calculated from its whole genome sequence was 44.6 mol%. Strain R14T contained menaquinone-7 and iso-C15:0, iso-C17:0 3-OH, C 16:0, C15:1 ω6c, and iso-C15:0-G as the sole respiratory isoprenoid quinone and major cellular fatty acids, respectively. Major polar lipids consisted of phosphatidylethanolamine, two unidentified aminolipids, and four unidentified lipids. Based on phylogenetic, phenotypic, and chemotaxonomic characteristics of strain R14T, it could be concluded that strain R14T represents a novel species of the genus Flavihumibacter, for which the name Flavihumibacter soli sp. nov. is proposed. The type strain is R14T (=KACC 21229T = JCM 33473T).
Introduction
Materials and Methods
The genus Flavihumibacter as a member of the family Chitinophagaceae within the phylum Bacteroidetes was first proposed by Zhang et al. with Flavihumibacter petaseus as the type species that was isolated from soil of a subtropical rainforest [1]. At the time of writing, the genus Flavihumibacter included five validly published species that have been isolated from soil [1–3] or sediment soil [4, 5]. In this study, one more putative novel species belonging to the genus Flavihumibacter was isolated from a soil sample and was taxonomically characterized using a polyphasic approach.
Isolation of Bacterial Strains
Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00284-020-02103-2) contains supplementary material, which is available to authorized users. The GenBank Accession Numbers for the 16S rRNA gene and genome sequences of strain R14T are MN086351 and JAAFZB000000000, respectively. * Che Ok Jeon [email protected] 1
Department of Life Science, Chung-Ang University, 84, HeukSeok‑Ro, Dongjak‑Gu, Seoul 06974, Republic of Korea
A soil sample for this study was collected from a steep grove in Chung-Ang University (37° 30′ 19.5″ N 126° 57′ 26.5″ E) located in Seoul of South Korea and strain R14T was isolated fr
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