Flavobacterium edaphi sp. nov., isolated from soil from Jeju Island, Korea
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ORIGINAL PAPER
Flavobacterium edaphi sp. nov., isolated from soil from Jeju Island, Korea Geeta Chhetri1 · Dahye Yang1 · Jiwon Choi1 · Hyungdong Kim1 · Taegun Seo1 Received: 27 June 2018 / Revised: 16 October 2018 / Accepted: 1 November 2018 © Springer-Verlag GmbH Germany, part of Springer Nature 2018
Abstract An aerobic, Gram-stain-negative, bright yellow-pigmented, oxidase and catalase-positive, non-motile, non-spore forming, rod-shaped strain designated D MN11T was isolated from the soil of crossroads of Jeju Island in South Korea. Colonies were circular, bright yellow-pigmented and smooth with regular edges and measured approximately 1–2 mm in diameter. Flexirubin-type pigments were absent. Phylogenetic tree analysis based on the 16SrRNA gene sequence revealed that the strain DMN11T formed a lineage within the family Flavobacteriaceae of the phylum Bacteroidetes, and it was the most closely related to Flavobacterium suzhouense XIN-1T and Flavobacterium hauense BX12T (98.6% and 98.2% similarity, respectively). The major isoprenoid quinone was MK-6. The major fatty acids were summed feature 3 (comprising C16:1 ω7c and/or C16:1 ω6c), iso-C15:0 and iso-C15:0 3OH. The polar lipid profile of the strain DMN11T showed the presence of phosphatidylethanolamine (PE) as major lipid. The DNA G+C content was 35.3 mol%, as determined by the thermal denaturation method. The mean levels of DNA–DNA relatedness of the strain DMN11T with F. suzhouense XIN-1T and F. hauense BX12T were 20.5% and 29.2%, respectively. Thus, the data accumulated in this study support the suggestion that the strain DMN11T is considered to represent a novel species of the genus Flavobacterieum, for which the name Flavobacterium edaphi sp. nov. is proposed. The type strain is DMN11T (= KCTC 62114T = JCM 32372T). Keywords Chemotaxonomic approach · Strictly aerobic · Flavobacteriaceae · DNA relatedness · Crossroad soil
Introduction The genus Flavobacterium (family Flavobacteriaceae, phylum Bacteroidetes) was first described by Bergey et al. (1957). It comprises 204 species (list of prokaryotic names with standing name in nomenclature: http://www.bacte rio.net/). Members of this genus have been isolated from various sources such as soil, freshwater, saltwater, glaciers, marine sediments and recently from faeces of the Antarctic penguins. Some strains of the genus Flavobacterium have attracted their scientific interest due to their ability to
express enzymes of biotechnological significance, thus making them potentially useful in bioremediation or wastewater treatment (Miyashita et al. 2010; Touchon et al. 2011; Huang et al. 2013). In addition, three Flavobacterium species are pathogenic to fish (Bernardet and Bowman 2006). DNA G+C content ranges from 30 to 41 mol%. The aim of the present study was to determine the taxonomic position of strain DMN11T by means of polyphasic approach. The phenotypic and genotypic characterizations of the novel strain are described in this report. The phylogenetic analyses indicated that strain DMN11T formed
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