Deinococcus detaillensis sp. nov., isolated from humus soil in Antarctica
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ORIGINAL PAPER
Deinococcus detaillensis sp. nov., isolated from humus soil in Antarctica Kun Zhang1 · Jing Zhu2 · Shuai Li3 · Manik Prabhu Narsing Rao3 · Ning‑Mei Li1 · Ang‑Yang Guo4 · Zhao Jiang5 · Qi‑Yong Tang2 · Yi Wan1 · Zhi‑Dong Zhang2 · Wen‑Jun Li3 Received: 27 February 2020 / Revised: 16 May 2020 / Accepted: 25 May 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A Gram-staining-positive, non-motile, coccus or short-rod-shaped bacterium, designated H 1T, was isolated from a humus soil sample in the Detaille Island of Antarctica. The 16S rRNA gene sequence result indicated that strain H 1T shared the highest 16S rRNA gene sequence identity with the type strain of Deinococcus alpinitundrae (96.2%). Growth of strain H 1T occurred at 4–25 °C, pH 6.0–8.0 and in the presence of 0–1.0% NaCl (w/v). The respiratory quinone was MK-8. The major fatty acids were C 16:0, C17:0 cyclo and summed feature 3 ( C16:1 ω7c/C16:1 ω6c). The polar lipids were aminoglycophospholipid, aminophospholipid, glycolipid and glycophospholipid. The cell wall peptidoglycan type was A3β. The genomic DNA G + C content was 61.3 mol%. The average nucleotide identity (ANI) between strain H 1T and the closely related Deinococcus members was below the cut-off level (95–96%) for species identification. Based on the above results, strain H1T represents a novel species of the genus Deinococcus, for which the name Deinococcus detaillensis sp. nov. is proposed. Type strain is H1T (= CGMCC 1.13938T = JCM 33291T). Keywords Humus soil · Polyphasic taxonomy · Deinococcus detaillensis Abbreviations ANI Average nucleotide identity AGPL Aminoglycophospholipid APL Aminophospholipid Communicated by Erko Stackebrandt. Kun Zhang, Jing Zhu and Shuai Li contributed equally to this work. The 16S rRNA gene sequence of strain H1T has been deposited in GenBank under the accession number MN116004. The GenBank/EMBL/DDBJ accession numbers for genome sequences of strain H1T and Deinococcus alpinitundrae LMG 24283T are VKDB00000000 and WXZL00000000, respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-01920-0) contains supplementary material, which is available to authorized users. * Zhi‑Dong Zhang [email protected] * Wen‑Jun Li [email protected] 1
Shaanxi Institute of Microbiology, Xi’an 710043, PR China
Xinjiang Laboratory of Special Environmental Microbiology, Institute of Microbiology, Xinjiang Academy of Agricultural Sciences, Urumqi 830091, PR China
2
GL Glycolipid GPL Glycophospholipid
Introduction The genus Deinococcus was first described by Brooks and Murray (1981). They have been isolated from different environments, such as soil (Dong et al. 2015; Joo et al. 2016; Kim et al. 2017), water (Im et al. 2008; Asker et al. 2009), desert (de Groot et al. 2005), hot spring (Ferreira et al. 1997), Antarctic environments (Hirsch et al. 2004), marine flatworm (Lin et al. 2017), car air-conditioning system (Kim et al. 2018), plants
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