Caenimonas soli sp. nov., isolated from soil
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ORIGINAL PAPER
Caenimonas soli sp. nov., isolated from soil Ram Hari Dahal1 · Hyosun Lee2 · Dhiraj Kumar Chaudhary3 · Dae‑Young Kim2 · Jigwan Son4 · Jaisoo Kim1 · Jong‑Ok Ka4 · Dong‑Uk Kim2 Received: 25 August 2020 / Revised: 7 October 2020 / Accepted: 23 October 2020 © Springer-Verlag GmbH Germany, part of Springer Nature 2020
Abstract A non-motile, Gram-stain-negative, rod-shaped bacterium, designated strain S4T, was obtained from soil sampled at Wonju, Gyeonggi-do, Republic of Korea. Cells were white-coloured, aerobic, grew optimally at 25–32 °C on R2A agar plate. A phylogenetic analysis based on its 16S rRNA gene sequence revealed that strain S 4T formed a lineage within the family T Comamonadaceae. The closest members were Caenimonas terrae SGM1–15 (98.1% sequence similarity), Caenimonas koreensis EMB320T (97.5%) and Ramlibacter solisilvae 5–10T (97.8%). The sequence similarities of strain S4T with other members of the family Comamonadaceae were ≤ 97.5%. The sole respiratory quinone was ubiquinone-8 (Q-8) and the principal polar lipid was phosphatidylethanolamine. The predominant cellular fatty acids were summed feature 3 (iso-C15 :0 2-OH/ C16 :1 ω7c), C16:0 and summed feature 8 (C18:1 ω7c and/or C 18:1 ω6c). The DNA G + C content was 65.1 mol%. In addition, the average nucleotide identity (ANIu) and in silico DNA–DNA hybridization (dDDH) relatedness values between strain S4T and Caenimonas koreensis were 77.6 and 21%, respectively. Based on genomic, chemotaxonomic, phenotypic, and phylogenetic analyses, strain S 4T represents a novel species in the genus Caenimonas, for which the name Caenimonas soli sp. nov. is proposed. The type strain is S4T (= KCTC 72742T = NBRC 114610T). Keywords Caenimonas soli · Proteobacteria · Comamonadaceae · Soil · Novel species
Introduction
Communicated by Erko Stackebrandt. Ram Hari Dahal and Hyosun Lee contributed equally to this study. GenBank/EMBL/DDBJ accessions of the 16S rRNA gene sequences for strain S4T is MN519471 and Whole Genome Shotgun project has been deposited at DDBJ/ENA/GenBank under the accession JABJVV000000000. The version described in this manuscript is version JABJVV 010000000. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-020-02110-8) contains supplementary material, which is available to authorized users. * Dong‑Uk Kim [email protected] 1
Department of Life Science, College of Natural Sciences, Kyonggi University, Suwon, Kyonggi‑Do 16227, Republic of Korea
Department of Biological Science, College of Science and Engineering, Sangji University, Wonju 26339, Republic of Korea
2
The genus Caenimonas was proposed by Ryu et al. for the description of Caenimonas koreensis isolated from activated sludge (Ryu et al. 2008) and the genus have been subsequently emended by Kim et al. (2012). To date, there are only two species with valid names, i.e. Caenimonas koreenis (Ryu et al. 2008) and Caenimonas terrae (Kim et al. 2012). The members of the genus Caenimonas were isolated fr
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