Bhargavaea changchunensis sp. nov. isolated from soil in China
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ORIGINAL PAPER
Bhargavaea changchunensis sp. nov. isolated from soil in China Feng‑Hua Tian1 · Dong‑Yu Fan1 · Chen Zhang1 · Chuan‑Wen Jia1 · Wei Gao2 · Yu Li1 · Chang‑Tian Li1 Received: 3 May 2018 / Revised: 12 August 2018 / Accepted: 20 August 2018 © Springer-Verlag GmbH Germany, part of Springer Nature 2018
Abstract A Gram-staining-positive, aerobic, rod-shaped (201802YP6T) bacteria was isolated from soil, Northeast of China. Growth occurred at 10–40 °C (optimum 25–30 °C), at pH 6.0–8.0 (optimum 7.0) and at 0–2% NaCl. Based on 16S rRNA gene sequence analysis, the nearest phylogenetic neighbors of strain 201802YP6T were identified as Bhargavaea cecembensis DSE10T (99.52%), Bhargavaea beijingensis ge10T (99.45%), Bhargavaea indica KJW98T (99.45%), Bhargavaea ullalensis ZMA19T (98.81%), and Bhargavaea ginsengi ge14T (98.76%). Levels of similarity among strain 201802YP6T and other Bhargavaea species were lower than 98.76%. GyrB amino acid sequence-based analysis supported the phylogenetic position and also distinguished strain 201802YP6T from the other species of the genus Bhargavaea. DNA–DNA hybridization values between strain 2 01802YP6T and B. cecembensis, B. beijingensis, B. indica, B. ullalensis, B. ginsengi were 43.5%, 43%, 32.5%, 30.5% and 20.4%, respectively. The DNA G + C content of strain 201802YP6T was 51.23 mol%. The average nucleotide identity (ANI) of the draft genome was 87.04% to B. cecembensis DSE10T. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipids, phosphatidylethanolamine, and phosphatidyllipid. The predominant menaquinone was MK-8. The major fatty acids were iso-C15:0 (39.91%), anteiso-C15:0 (28.86%), anteiso-C17:0 (6.30%) and C16:0 (6.13%). On the basis of the phylogenetic analysis, chemotaxonomic data, physiological characteristics and DNA–DNA hybridization data, strain 201802YP6T represents a novel species of the genus Bhargavaea, for which the name Bhargavaea changchunensis sp. nov. is proposed. The type strain is 201802YP6T (= CGMCC 1.13508T = KCTC 33975T). Keywords Bhargavaea changchunensis sp. nov. · Polyphasic analysis · 16S rRNA gene · Phenotypic characteristics · Taxonomy · Genome sequencing
Introduction Communicated by Erko Stackebrandt. The NCBI GenBank accession numbers for the 16S rRNA and genome sequence of strain 201802YP6T are MH094655 and SUB4396199 (submission), respectively. Electronic supplementary material The online version of this article (https://doi.org/10.1007/s00203-018-1563-6) contains supplementary material, which is available to authorized users. * Yu Li [email protected] * Chang‑Tian Li [email protected] 1
Engineering Research Center of Edible and Medicinal Fungi, Jilin Agricultural University, Ministry of Education, Changchun 130118, People’s Republic of China
Tonghua Edible Fungus Cultivation Cooperatives, Tonghua 134100, People’s Republic of China
2
The genus Bhargavaea was first proposed and described by Manorama et al. (Manorama et al. 2009; Verma et al. 2012), and is a member of the family Pl
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