Hypoxia Methods and Protocols
This volume details widely used and newer lab protocols for studying hypoxic responses in physiology and diseases. Chapters guide the reader through the application of hypoxic conditions, to the techniques of molecular biology, bi
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L. Eric Huang Editor
Hypoxia Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Hypoxia Methods and Protocols
Edited by
L. Eric Huang Department of Neurosurgery, Clinical Neurosciences Center, University of Utah, Salt Lake City, UT, USA
Editor L. Eric Huang Department of Neurosurgery Clinical Neurosciences Center University of Utah Salt Lake City, UT, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-7664-5 ISBN 978-1-4939-7665-2 (eBook) https://doi.org/10.1007/978-1-4939-7665-2 Library of Congress Control Number: 2017963643 © Springer Science+Business Media, LLC 2018 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media, LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Oxygen is an essential molecule that serves as an enzyme substrate in bioenergetics and metabolic reactions. Adaptation to oxygen deprivation—hypoxia—is a critical process in development, physiology, and pathophysiology. This volume offers a comprehensive coverage of step-by-step lab protocols that are widely used and those that are developed more recently for studying hypoxic responses in physiology and diseases. All the protocols contain extensive detail intended to help competent scientists in the field of biomedical sciences carry out unfamiliar molecular biology techniques successfully by simply following the detailed procedures. Practical notes are explained in greater detail for additional help. Methods included range from the application of hypoxic conditions t
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