Inositol Phosphates and Lipids Methods and Protocols
Inositol, in its native or lipid derived forms, serves as a master building block which, when phosphorylated, leads to the construction of more than 30 unique isomeric forms that are employed in vital but diverse regulatory roles in cells. In Inositol Pho
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Molecular Biology™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
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Inositol Phosphates and Lipids Methods and Protocols
Edited by
Christopher J. Barker The Rolf Luft Research Center for Diabetes and Endocrinology, Karolinska Institutet, Stockholm, Sweden
Editor Christopher J. Barker The Rolf Luft Research Center for Diabetes and Endocrinology Karolinska Institutet Stockholm, Sweden [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-60327-174-5 e-ISBN 978-1-60327-175-2 DOI 10.1007/978-1-60327-175-2 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2010927804 © Springer Science+Business Media, LLC 2010 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. While the advice and information in this book are believed to be true and accurate at the date of going to press, neither the authors nor the editors nor the publisher can accept any legal responsibility for any errors or omissions that may be made. The publisher makes no warranty, express or implied, with respect to the material contained herein. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface This book seeks to cover a broad range of techniques encountered by those working within the field of phosphorylated inositols. This field started with the observation by the Hokins of the turnover of phosphatidylinositol, a minor membrane lipid, in response to agonists. However, it was more than 20 years before Michell proposed a hypothesis suggesting a functional link between this lipid turnover and intracellular calcium homeostasis that spurred a new wave of interest, cumulating with the description of IP3 as a second messenger by Berridge, Irvine, and their colleagues. This was followed by the later discovery of phosphatidylinositol 3 kinase by Cantley and co-workers. These significant advances were, however, just the beginning of a field that now encompasses every conceivable area of cell biology. The all-pervasiveness of these compounds in cellular regulation is largely facilitated by the unique structure of inositol which, when phosphorylated either in its native or lipid-derived forms, leads to the production of more th
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