Legionella Methods and Protocols
The methods described in the second edition on Legionella are for the study of distinct features of L. pneumophila. Chapters guide readers through ecology and physiology of legionella, legionella genetics, cellular microbiology of legionella, bioche
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Carmen Buchrieser Hubert Hilbi Editors
Legionella Methods and Protocols Second Edition
METHODS
IN
MOLECULAR BIOLOGY
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Legionella Methods and Protocols Second Edition
Edited by
Carmen Buchrieser Institut Pasteur, Biologie des Bactéries Intracellulaires, Paris, France CNRS UMR 3525, Paris, France
Hubert Hilbi Institute of Medical Microbiology, University of Zürich, Zürich, Switzerland
Editors Carmen Buchrieser Institut Pasteur, Biologie des Bacte´ries Intracellulaires Paris, France
Hubert Hilbi Institute of Medical Microbiology University of Zu¨rich Zu¨rich, Switzerland
CNRS UMR 3525 Paris, France
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9047-4 ISBN 978-1-4939-9048-1 (eBook) https://doi.org/10.1007/978-1-4939-9048-1 Library of Congress Control Number: 2018967004 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover illustration: Human primary macrophages (hMDMs) infected with Legionella pneumophila (green) stained with mitotracker (red) visualizing the mitochondrial network of the hMDM during infection Photo credit: Pedro Escoll, Institut Pasteur, Biology of Intracellular Bacteria, 75724 Paris, France This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Summary Legionella species are the causative agents of a life-threatening form of pneumonia called Legionnaires’ disease, which was identified more than 40 years ago. The opportunistic pathogens can occur in epidemics of several hundreds
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