Leishmania Methods and Protocols
This volume explores the latest techniques used to study Leishmania and other trypanosomatidae. Chapters in this book cover a wide range of topics such as cultivation of axenic amastigote forms; phylogeny and comparative genomics; and in vitro and in vivo
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Joachim Clos Editor
Leishmania Methods and Protocols
Methods
in
M o l e c u l a r B i o lo g y
Series Editor: John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Leishmania Methods and Protocols
Edited by
Joachim Clos Bernhard Nocht Institute for Tropical Medicine , Hamburg, Germany
Editor Joachim Clos Bernhard Nocht Institute for Tropical Medicine Hamburg, Germany
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-9209-6 ISBN 978-1-4939-9210-2 (eBook) https://doi.org/10.1007/978-1-4939-9210-2 Library of Congress Control Number: 2019935497 © Springer Science+Business Media, LLC, part of Springer Nature 2019 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors, and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Cover Caption: Scanning electron micrograph of a Leishmania donovani promastigote, taken by Dr. Monica Hagedorn at the electron microscope facility of the University of Ulm, Germany. This Humana Press imprint is published by the registered company Springer Science+Business Media, LLC, part of Springer Nature. The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface The Aims Parasites of the genus Leishmania and other Trypanosomatidae are causative for three of the most important neglected tropical diseases (NTDs), affecting the lives of millions of humans in the tropical and subtropical regions of the world. New drugs against these obligate parasites and new tools for the control of their vectors are urgently needed, especially since all existing treatments have serious drawbacks and limitations. Therefore, additional efforts are needed; meaning, more researchers must enter the field, not only in applied science but also in basic research. This is often hampered by a lack of understanding of these o
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