Lipidomics Methods and Protocols

This volume explores lipidomics through protocols that focus on areas of utility, techniques, and bioinformatics advancements. The protocols in this book cover topics such as isolation of specific membranes and specialized fractionation of subcellular com

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Sanjoy K. Bhattacharya Editor

Lipidomics Methods and Protocols

Methods

in

Molecular Biology

Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK

For further volumes: http://www.springer.com/series/7651

Lipidomics Methods and Protocols

Edited by

Sanjoy K. Bhattacharya University of Miami, Miami, FL, USA

Editor Sanjoy K. Bhattacharya University of Miami Miami, FL, USA

ISSN 1064-3745     ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6995-1    ISBN 978-1-4939-6996-8 (eBook) DOI 10.1007/978-1-4939-6996-8 Library of Congress Control Number: 2017939352 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.

Preface The history of lipids spans almost four centuries, ever since Techenius Otto suggested the presence of acidic compound in fat in 1673 (fatty acids: Otto found that the alkali is neutralized by animal fat in the process of making soap). Lipids are the biomolecules that provide boundary to cells. They are also highly efficient signaling molecules. Every fragment generated from a lipid can be used for differential signaling in a living cell. Indeed, all major journals with biological chemistry mandate have a dedicated section for lipid biochemistry and signaling. Despite a rich history of lipid research that includes perspectives from fundamental biology and synthetic organic chemistry, the lipids are not a dominant theme in most biological laboratories. Only about a 10th or less of the laboratories engaged in biomedical research focus on lipid biochemistry. This is also partly due t