Morpholino Oligomers Methods and Protocols
This volume presents a historical perspective on Morpholinos, an overview of good Morpholino practices, techniques for controlling Morpholino activity with light, techniques for modulating microRNA activity in zebrafish embryos, probing genes during fin r
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Hong M. Moulton Jon D. Moulton Editors
Morpholino Oligomers Methods and Protocols
Methods
in
Molecular Biology
Series Editor John M. Walker School of Life and Medical Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Morpholino Oligomers Methods and Protocols
Edited by
Hong M. Moulton Department of Biomedical Sciences, College of Veterinary Medicine, Oregon State University, Corvallis, OR, USA
Jon D. Moulton Gene Tools, LLC, Philomath, OR, USA
Editors Hong M. Moulton Department of Biomedical Sciences College of Veterinary Medicine Oregon State University Corvallis, OR, USA
Jon D. Moulton Gene Tools, LLC Philomath, OR, USA
ISSN 1064-3745 ISSN 1940-6029 (electronic) Methods in Molecular Biology ISBN 978-1-4939-6815-2 ISBN 978-1-4939-6817-6 (eBook) DOI 10.1007/978-1-4939-6817-6 Library of Congress Control Number: 2017937324 © Springer Science+Business Media LLC 2017 This work is subject to copyright. All rights are reserved by the Publisher, whether the whole or part of the material is concerned, specifically the rights of translation, reprinting, reuse of illustrations, recitation, broadcasting, reproduction on microfilms or in any other physical way, and transmission or information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed. The use of general descriptive names, registered names, trademarks, service marks, etc. in this publication does not imply, even in the absence of a specific statement, that such names are exempt from the relevant protective laws and regulations and therefore free for general use. The publisher, the authors and the editors are safe to assume that the advice and information in this book are believed to be true and accurate at the date of publication. Neither the publisher nor the authors or the editors give a warranty, express or implied, with respect to the material contained herein or for any errors or omissions that may have been made. The publisher remains neutral with regard to jurisdictional claims in published maps and institutional affiliations. Printed on acid-free paper This Humana Press imprint is published by Springer Nature The registered company is Springer Science+Business Media LLC The registered company address is: 233 Spring Street, New York, NY 10013, U.S.A.
Preface Morpholino phosphorodiamidate antisense oligos (Morpholinos) were originally developed for therapeutic applications but have found widespread use as research reagents. They are readily available, easy-to-handle, and stable oligos that bind to complementary RNA and prevent other large molecules from accessing their bound target, like sequence-specific masking tape for RNA. Their function depends on the usual biological function of their target sequence. They have been used in animals, bacteria, protists, plants, and fungi. They don’t rely on the cells for enzymatic activity such as RNase-H or RISC. Instead the oligos s
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