Mucins Methods and Protocols
Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. In, Mucins: Methods and Protocols expert researchers in the field detail many of the methods which are now commonly used to study Mucin
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IN
MOLECULAR BIOLOGY™
Series Editor John M. Walker School of Life Sciences University of Hertfordshire Hatfield, Hertfordshire, AL10 9AB, UK
For further volumes: http://www.springer.com/series/7651
Mucins Methods and Protocols Edited by
Michael A. McGuckin Immunity, Infection and Inflammation Program, Mater Medical Research Institute, South Brisbane, QLD, Australia
David J. Thornton Wellcome Trust Centre for Cell-Matrix Research, Faculty of Life Sciences, University of Manchester, Manchester, UK
Editors Michael A. McGuckin Immunity, Infection and Inflammation Program Mater Medical Research Institute South Brisbane, QLD, Australia [email protected]
David J. Thornton Wellcome Trust Centre for Cell-Matrix Research Faculty of Life Sciences University of Manchester Manchester, UK [email protected]
ISSN 1064-3745 e-ISSN 1940-6029 ISBN 978-1-61779-512-1 e-ISBN 978-1-61779-513-8 DOI 10.1007/978-1-61779-513-8 Springer New York Dordrecht Heidelberg London Library of Congress Control Number: 2011944359 © Springer Science+Business Media, LLC 2012 All rights reserved. This work may not be translated or copied in whole or in part without the written permission of the publisher (Humana Press, c/o Springer Science+Business Media, LLC, 233 Spring Street, New York, NY 10013, USA), except for brief excerpts in connection with reviews or scholarly analysis. Use in connection with any form of information storage and retrieval, electronic adaptation, computer software, or by similar or dissimilar methodology now known or hereafter developed is forbidden. The use in this publication of trade names, trademarks, service marks, and similar terms, even if they are not identified as such, is not to be taken as an expression of opinion as to whether or not they are subject to proprietary rights. Printed on acid-free paper Humana Press is part of Springer Science+Business Media (www.springer.com)
Preface Introduction to Mucin Biology and Technical Challenges of Mucin Research Epithelial mucins are large complex cell surface and secreted glycoproteins produced by mucosal epithelial cells. Mucins are a major component of the interface between the external world and mucosal tissues, where they provide lubrication, hydration, and a biological and physical barrier to potential toxins, particles, and pathogens. Mucins provide many challenges to researchers due to their large size, complex biochemical nature, and the viscous gels that they form when secreted. Overcoming these challenges is centrally important to a full understanding of mucosal biology and the contribution of mucins to normal human physiology and disease. In this volume of the Methods in Molecular Biology series, we have highlighted the technical challenges while describing procedures that are specifically relevant to the analysis of mucins and their contribution to mucosal biology. We have gathered a group of experts together to overview the best approaches to analysing each specific area of mucin biochemistry, physiology, and biophysics before provi
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